CD25/IL-2R alpha Antibody (PC61)

Product Discontinued

CD25/IL-2R alpha Antibody (PC61) Summary

• Immunogen: The B6.1 mouse cytotoxic T cell line was used as immunogen for this antibody.
• Isotype: IgG1 Lambda
• Clonality: Monoclonal
• Host: Rat
• Gene: IL2RA
• Purity: Protein G purified
• Endotoxin Note: < 0.06 EU/ug

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry 0.1 - 0.5 ug/10^6cells
  • In vivo assay
• Application Notes: Mouse splenocytes were stimulated with 5 ug/mL ConA for three days prior to testing. Use in In vivo assay reported in scientific literature (PMID 26740625).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Sterile PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CD25/IL-2R alpha Antibody (PC61)

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

CD25 is a 55kDa transmembrane glycoprotein consisting of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. It is expressed during early stages of thymocytes development and in activated CD4+ memory T-lymphocytes. CD25 heterodimerizes with IL2R-beta and IL-2R gamma and forms the tripartite high affinity IL-2 receptor complex. It increases the specificity and affinity of IL-2 receptor complex for IL-2 ligand and is necessary for receptor clustering required for augmentation of IL-2 signaling. Mutations in CD25 result in severe immunodeficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD25/IL-2R alpha Antibody (NBP2-27425)(4)

Publications using NBP2-27425

• Salman S, Meyers DJ, Wicks EE Et al. HIF inhibitor 32-134D eradicates murine hepatocellular carcinoma in combination with anti-PD1 therapy J Clin Invest 2022-05-02 [PMID: 35499076]
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130]
  
  Details:
  Citation using the PE format of this antibody.
• Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018-01-24 [PMID: 29367423] (FLOW, Mouse)
  
  Details:
  This citation used the Alexa Fluor 488 form of this antbody
• Clement CC, Becerra A, Yin L et al. The Dendritic Cell MHC II Peptidome Derives from a Variety of Processing Pathways and Includes Peptides with a Broad Spectrum of HLA-DM Sensitivity. J. Biol. Chem. 2016-01-06 [PMID: 26740625] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: IL2RA
• Uniprot:
  • P01589()