CD3 epsilon Antibody (C363.29B (YCD3-1)) - Azide and BSA Free

Flow Cytometry: CD3 epsilon Antibody (C363.29B (YCD3-1)) [NBP1-26685] - Analysis using the PE conjugate of NBP1-26685. Multiple staining of BALB/c splenocytes.

Flow Cytometry: CD3 epsilon Antibody (C363.29B (YCD3-1)) [NBP1-26685] - Analysis using the FITC conjugate of NBP1-26685. Double staining of 3 ug/1^6 Cells from BALB/c mesenteric lymph nodes with anti-mouse CD3 epsilon-FITC and anti-mouse CD45R/B220-R-PE, gated on small lymphocytes.

Product Discontinued

CD3 epsilon Antibody (C363.29B (YCD3-1)) - Azide and BSA Free Summary

• Immunogen: IL-4 producing Th2 cell lines including D10
• Specificity: Mouse CD3 epsilon (Mr 25 kD)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CD3E
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Flow Cytometry, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro depletion of CD3+ cells, In vitro activation of T cells (accessory cells must be provided)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD3 epsilon Antibody (C363.29B (YCD3-1)) - Azide and BSA Free

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains.(1, 2) CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.(1) In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naive T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present.(1, 3, 4) The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody.(1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD3 epsilon Antibody (NBP1-26685)(6)

Publications using NBP1-26685

• Tremble LF, Heffron CCBB, Forde PF The effect of calcium electroporation on viability, phenotype and function of melanoma conditioned macrophages Sci Rep 2020-11-26 [PMID: 33244152] (FLOW)
• WhitelJL, Nicholls SM, Shimeld C et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies J Histochem Cytochem 1995-03-01 [PMID: 7868861]
  
  Details:
  This citation used the Biotin version of this antibody.
• Portoles P, Rojo J, Golby A et al. Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation J Immunol 1989-06-01 [PMID: 2470817]
  
  Details:
  This citation used the Biotin version of this antibody.
• Dianzani U, Luqman M, Rojo J et al. Molecular associations on the T cell surface correlate with immunological memory Eur J Immunol 1990-10-01 [PMID: 1978709]
  
  Details:
  This citation used the Biotin version of this antibody.
• Ernst DN, Hobbs MV, Torbett BE et al. Differences in the expression profiles of CD45RB, Pgp-1, and 3G11 membrane antigens and in the patterns of lymphokine secretion by splenic CD4+ T cells from young and aged mice J Immunol [PMID: 1974562]
  
  Details:
  Used the Biotin form of this antibody.
• Luqman M, Greenbaum L, Lu D et al. Differential effect of interleukin 1 on naive and memory CD4+ T cells Eur J Immunol 1992-01-01 [PMID: 1346115]
  
  Details:
  This citation used the Biotin version of this antibody.

Bioinformatics

• Gene Symbol: CD3E