CD31/PECAM-1 Antibody (CO.3E1D4) - BSA Free

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Valvular endothelial cell (VEC) markers. Sections of the sheep aortic valve leaflet stained with antibodies against the endothelial cell markers CD31/PECAM-1, CD34, VE-Cadherin (CD144), or von Willebrand Factor (vWF). Represented are the aortic wall and a section of the valvular leaflet (near the free edge), with

Flow Cytometry: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Staining of sheep peripheral blood platelets with Mouse anti Bovine CD31/PECAM-1 Antibody (CO.3E-1D4).

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Bovine Lung stained with CD31 (green) and DAPI counterstain. Image provided courtesy of Hala Nijmeh, PhD and the PI, Evgenia V. Gerasimovskaya, PhD. Section of Critical Care Medicine, Department of Pediatrics, University of Colorado Anschutz Medical Center.

• Reactivity: Sh
• Applications: Flow, IHC, IP
• Clone: CO.3E1D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CD31/PECAM-1 Antibody (CO.3E1D4) - BSA Free Summary

• Immunogen: Ovine leucocytes
• Specificity: NB100-65900 is specific for ovine CD31, also known as PECAM-1. Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes. CD31 is also highly expressed by ovine endothelial cells.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PECAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:25
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Frozen 1:50
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
• Application Notes: Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 30159315).
  Clone CO.3E1D4 is reported to inhibit homotypic leucocyte aggregation induced by anti CD43 antibodies. Removal of sodium azide is recommended prior to use in fuctional assays.
• Theoretical MW: 82.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted cross-reactivities: Goat, Bovine

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for CD31/PECAM-1 Antibody (CO.3E1D4) - BSA Free

• adhesion molecule
• CD31 antigen
• CD31
• CD31/EndoCAM
• EndoCAM
• FLJ34100
• FLJ58394
• GPIIA'
• PECA1
• PECAM1
• PECAM-1
• PECAM-1, CD31/EndoCAM
• platelet endothelial cell adhesion molecule
• platelet endothelial cell adhesion molecule-1
• platelet/endothelial cell adhesion molecule

Background

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD31/PECAM-1 Antibody (NB100-65900)(6)

Publications using NB100-65900

• Collie D, Wright SH, Del-Pozo J et al. Regional and organ-level responses to local lung irradiation in sheep Scientific reports 2021-05-05 [PMID: 33953285] (IF/IHC, Bovine)
• Bourebaba L, Kornicka-Garbowska K, Al Naem M et al MSI-1436 improves EMS adipose derived progenitor stem cells in the course of adipogenic differentiation through modulation of ER stress, apoptosis, and oxidative stress Stem Cell Res Ther 2021-02-04 [PMID: 33536069] (FLOW, Equine)
  
  Details:
  Citation using the Allophycocyanin version of this antibody.
• Dekker S, van Geemen D, van den Bogaerdt AJ et al. Sheep-Specific Immunohistochemical Panel for the Evaluation of Regenerative and Inflammatory Processes in Tissue-Engineered Heart Valves Front Cardiovasc Med 2018-08-15 [PMID: 30159315] (IHC-P, Sheep)
• Lu S, McGough MAP, Shiels SM et al. Settable polymer/ceramic composite bone grafts stabilize weight-bearing tibial plateau slot defects and integrate with host bone in an ovine model. Biomaterials. 2018-10-01 [PMID: 29960822] (IF/IHC, Sheep)
• Kluin J, Talacua H, Smits AI et al. In situ heart valve tissue engineering using a bioresorbable elastomeric implant - From material design to 12 months follow-up in sheep. Biomaterials. 2017-05-01 [PMID: 28253994]
• Nijmeh H, Balasubramaniam V, Burns N et al. High proliferative potential endothelial colony-forming cells contribute to hypoxia-induced pulmonary artery vasa vasorum neovascularization Am. J. Physiol. Lung Cell Mol. Physiol. 2014-04-01 [PMID: 24508729] (IHC-Fr, Bovine)
  
  Details:
  CD31/PECAM-1 antibody used at 1:50 dilution in IHC-Fr application for 4% paraformaldehyde fixed frozen sections of lung tissue and pulmonary arteries that were collected from male Holstein calves subjected to normoxic (kept at ambient Denver 91 altitude; PB = 640 mmHg for 2 weeks) and chronically hypoxic (exposed to hypobaric hypoxia; PB = 430 92 mmHg for 2 weeks). Figure 1d-i shows the immunostaining of endothelial cells/CD31+ve in main pulmonary artery/MPA vasa vasorum/VV sections from control and chronically hypoxic animals.

Bioinformatics

• Gene Symbol: PECAM1