CD35 Antibody (E11)

Immunohistochemistry-Paraffin: CD35 Antibody (E11) [NBP2-29460] - Hhuman Tonsil stained with CD35 Antibody (E11).

Immunohistochemistry-Paraffin: CD35 Antibody (E11) [NBP2-29460] - Hhuman Tonsil stained with CD35 Antibody (E11).

• Reactivity: Hu, Pm, Pm, Pm-Cm, RM
• Applications: Flow, Func, ICC/IF, IHC
• Clone: E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD35 Antibody (E11) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33080)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Additional Information: Clone E11 was used by HLDA to establish CD designation.
• Immunogen: Intact human monocytes
• Localization: Cell surface
• Marker: Follicular Dendritic Cell Marker
• Specificity: Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This monoclonal antibody is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This monoclonal antibody is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This monoclonal antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CR1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • In-situ Hybridization
• Application Notes: Use in In-situ Hybridization reported in scientific literature (PMID:34855915).Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined..

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD35 Antibody (E11)

• C3BR
• CD35
• complement component (3b/4b) receptor 1 (Knops blood group)
• CR1
• KN

Background

The clone E11 antibody reacts with CD35, also known as complement receptor 1 (CR1), a 210-220 kDa protein (Hogg et al, 1985). CD35 is present on a subpopulation of T lymphocytes (~5%) which are relatively low in CD3 expression. CD35 is also expressed on CD20 positive B lymphocytes, CD14 positive monocytes, and on poly-morphonuclear leukocytes. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. In Immunohistochemical staining (IHC) the CD35 antibody strongly labels the follicular dendritic cells (FDCs) and follicular dendritic cell sarcoma. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. The antibody is highly specific for CD35 and does not cross react with CD21 (CR2). Furthermore, this CD35 antibody does not block CD35 activity because it recognizes a CD35 epitope distal to the C3b binding site. The antibody has been widely cited in the literature. Examples include its use in antibody panels for immunophenotyping normal bone marrow stem cells by flow cytometry (Laranjeira et al, 2012) and characterizing infections in rheumatoid arthritis patients (Moduko et al, 2012). The CD35 antibody has also been used along with CD23 and CD21 antibodies for identifying FDCs in reactive and neoplastic lymphoid tissue by IHC (Bagdi et al, 2001). Furthermore, the CD35 antibody showed than tonsil-derived mesenchymal progenitor cells acquire an FDC-like phenotype following cytokine stimulation (Cho et al, 2012), and that oncolytic measles viral infection upregulates CD35 (Zhang et al, 2012). Additional publications using the CD35 antibody can be identified through a key word on scholar.google.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD35 Antibody (NBP2-29460)(6)

Publications using NBP2-29460

• Broeckel RM, Feldmann F, McNally KL et al. A pigtailed macaque model of Kyasanur Forest disease virus and Alkhurma hemorrhagic disease virus pathogenesis PLoS pathogens 2021-12-01 [PMID: 34855915] (ISH, Monkey)
• Dave Rajnish S, Sharma Ravi K, Muir Roshell R et al FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques. J Neuroimmune Pharmacol. 2018-06-01 [PMID: 29288344] (IHC-P, Monkey)
  
  Details:
  Citation using the HRP version of this antibody.
• Cho KA, Kim JY, Kim HS et al. Tonsil-derived mesenchymal progenitor cells acquire a follicular dendritic cell phenotype under cytokine stimulation. Cytokine. 2012-08-01 [PMID: 22578801]
• Mokuda S, Doi O, Takasugi K. Simultaneous quantitative analysis of the expression of CD64 and CD35 on neutrophils as markers to differentiate between bacterial and viral infections in patients with rheumatoid arthritis. Mod Rheumatol. 2012-09-01 [PMID: 22237765]
• Zhang Y, Patel B, Dey A et al. Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function. J Immunol. 2012-02-01 [PMID: 22180616]
• Bagdi E, Krenacs L, Krenacs T et al. Follicular dendritic cells in reactive and neoplastic lymphoid tissues: a reevaluation of staining patterns of CD21, CD23, and CD35 antibodies in paraffin sections after wet heat-induced epitope retrieval. Appl Immunohistochem Mol Morphol. 2001-06-01 [PMID: 11396628]

Bioinformatics

• Gene Symbol: CR1
• Uniprot:
  • P17927(Human)