CD44 Antibody (5035-41.1D) - BSA Free

Flow Cytometry: CD44 Antibody (5035-41.1D) [NB100-65905] - Analysis using the FITC conjugate of NB100-65905. Staining of mouse spleen cells.

Flow Cytometry: CD44 Antibody (5035-41.1D) [NB100-65905] - Mouse spleen cells.

• Reactivity: Mu
• Applications: Flow, IP
• Clone: 5035-41.1D
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD44 Antibody (5035-41.1D) - BSA Free Summary

• Immunogen: Spleen cells from B6PL-Ly-2a / Ly-3a B6-Ly-1a mice
• Marker: Cell Membrane Marker
• Specificity: This recognizes the following strains of mice:- C57BL/6, C57BL/10, C57/L, C58A, AKR, 129, SJL, NZB, C3H, CE, CBA/H.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD44
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Human reactivity reported in scientific literature (PMID:32901011)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CD44 Antibody (5035-41.1D) - BSA Free

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD44 Antibody (NB100-65905)(3)

Publications using NB100-65905

• Shikanai M, Ito S, Nishimura YV et al. Rab21 regulates caveolin-1-mediated endocytic trafficking to promote immature neurite pruning EMBO reports 2023-03-06 [PMID: 36683567] (Immunocytochemistry/ Immunofluorescence, Mouse)
• FitzGerald LI, Aurelio L, Chen M et al. A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells Nature communications 2020-09-08 [PMID: 32901011] (ICC/IF, Human)
• Chaudhary N, Gomez GA, Howes MT et al. Endocytic crosstalk: cavins, caveolins, and caveolae regulate clathrin-independent endocytosis. PLoS Biol. 2014-04-01 [PMID: 24714042] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: CD44
• Entrez:
  • 12505(Mouse)
• Uniprot:
  • P15379(Mouse)