Human CD44 - variant 5 ELISA Kit (Colorimetric)

Sandwich ELISA: Human CD44, variant 5 ELISA Kit (Colorimetric) [NBP1-89874] - Representative standard curve for human CD44var (v5) ELISA. Human CD44var (v5) was diluted in serial 2-foldsteps in Sample Diluent. Do not use this standard curve to derive test results. A standard curve must be run for eachgroup of microwell strips assayed.

Product Discontinued

Human CD44 - variant 5 ELISA Kit (Colorimetric) Summary

• Description: The human sCD44var (v5) ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD44var (v5)
• Specificity: This assay recognizes both natural and recombinant forms of the CD44var (v5) molecule. The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human CD44var (v5) positive serum. There was no crossreactivity detected, namely not with human CD44 polypeptides lacking the protein sequence encoded by exon 5
• Standard Curve Range: 0.31 - 10.0 ng/mL
• Sensitivity: 0.14 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.8%
• Intra-Assay: CV% < 3.6%
• Spike Recovery: The recovery ranged from 84% to 100% with an overall mean recovery of 92%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 20 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CD44

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CD44 - variant 5 ELISA Kit (Colorimetric)

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CD44