CD45 Antibody (30-F11) - BSA Free

Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with [NB100-77417] at 1:1000 dilution and detected with FITC conjugated donkey anti-rat IgG secondary (orange). Shown with rat IgG isotype control (blue).

Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with [NB100-77417] at 1:1000 dilution and detected with FITC conjugated donkey anti-rat IgG secondary (orange). Shown with rat IgG isotype control (blue).

Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Analysis of FFPE section of mouse spleen using rat monoclonal CD45 antibody (30-F11) [NB100-77417] at 1:50 dilution. The antibody generated an expected membrane staining in the red and white pulp spleenocytes of the tested spleen section.

Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Mouse glomeruli stained with CD45 antibody. 4% formaldehyde fixed paraffin sections. EDTA was used for antigen retrieval. IHC-P image submitted by a verified customer review.

Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a FFPE embedded tissue section of mouse liver using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. The signal was developed using AEC reagent based method with hematoxylin counterstaining.

Immunohistochemistry-Frozen: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a mouse spleen cryosection using CD45 antibody (30-F11) [NB100-77417] at 1:50 dilution. The signal was detected using goat anti-rat Alexa fluor 488 secondary with DAPI counterstaining. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Staining of a FFPE mouse liver tissue section using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. Signal was detected using AF488 conjugated donkey anti-rat secondary antibody at 1:500 dilution. IHC-P image submitted by a verified customer review.

Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Severe apoptosis and pathology in the orally infected heart. Experimental design is as outlined in Fig. 1B. Heart sections from moribund mice were examined with H&E, and immunohistochemistry staining for pan-leukocyte infiltration using an anti-CD45 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.

Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Severe apoptosis and pathology in the orally infected heart. Experimental design is as outlined in Fig. 1B. Heart sections from moribund mice were examined with H&E, and immunohistochemistry staining for pan-leukocyte infiltration using an anti-CD45 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.

Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - VP1-positive infiltrating M2 macrophage and VP1-positive pneumocytes were detectable in the orally infected lung. Adjacent lung sections were examined with H&E and IHC staining for a pan-leukocyte marker CD45. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, Func, ICC/IF, IHC, IP, CyTOF-ready
• Clone: 30-F11
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CD45 Antibody (30-F11) - BSA Free Summary

• Immunogen: This CD45 Antibody (30-F11) was prepared from Mouse thymus or spleen.
• Localization: Transmembrane Protein with Extracellular, Helical and Cytoplasmic Domains
• Specificity: Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: PTPRC
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Functional reported in scientific literature (PMID 7743522)
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • In vitro assay reported in scientific literature (PMID 1974916)
  • In vivo assay reported in multiple pieces of scientific literature
  • Western Blot 1:100-1:2000
• Application Notes: Each lot of this CD45 antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. IF data from customer review. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. It is recommended that the reagent be titrated for optimal performance for each application. This antibody is CyTOF ready. NB100-77417 may also be used for IHC with acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections.
• Theoretical MW: 145 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Human reported in scientific literature (PMID:34494385).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CD45 Antibody (30-F11) - BSA Free

• B220
• CD_antigen: CD45
• CD45 antigen
• CD45
• CD45R
• EC 3.1.3.48
• EC:3.1.3.48
• GP180
• LCA
• L-CA
• Leukocyte common antigen
• LY5
• protein tyrosine phosphatase, receptor type, c polypeptide
• PTPRC
• receptor-type tyrosine-protein phosphatase C
• T200 Glycoprotein
• T200 leukocyte common antigen
• T200

Background

CD45, also known as leukocyte common antigen (LCA), T200, or Ly5, is a member C of the class 1 receptor-like protein tyrosine phosphatase (PTPRC) family (1, 2). It is a transmembrane glycoprotein which, due to alternative splicing, has a multiple isoforms with a theoretical molecular weight ranging from 180 - 220 kDa (1, 3-5). Human CD45 is synthesized as a 1281 amino acid sequence consisting of an alternatively spliced extracellular receptor-like region, a cysteine-rich domain, fibronectin-like III repeats, a transmembrane segment, and a cytoplasmic region with tandem protein tyrosine phosphatase (PTP) domains: the membrane proximal domain (D1) and the membrane distal domain (D2) (3, 5). CD45 is expressed on all nucleated hematopoietic cells and their precursors, except mature red blood cells, and is one of the most abundantly-expressed cell-surface glycoproteins, comprising approximately 10% of surface proteins in lymphocytes (3). Functionally, CD45 is essential for development and activation of T-cells and B-cells (1-5). More specifically, CD45 positively regulates antigen receptor signaling and Src-family member kinase activity (1, 3). There are many ways to regulate CD45 phosphatase activity including ligand binding, dimerization, protein interactions, cellular localization, and covalent modifications (3, 6). Ligands for CD45 include pUL11, a transmembrane protein of the cytomegalovirus RL11 (CMV RL11) family, and placental protein 14 (PP14), both of which exclusively bind CD45, and various lectins including CD22, galectin-1, galectin-3, macrophage mannose receptor (MR), and macrophage galactose-type lectin (MGL) (6).

Given its role in immune cell development and activation, CD45 has also been linked to a variety of diseases. The importance of CD45 in immunity has been revealed in human and mouse studies where CD45-deficiency leads to a severe-combined immunodeficiency (SCID) phenotype (2, 3, 6). A CD45-knockout mice study revealed inhibited thymocyte production and poor B-cell response, whereas CD45 activation in mice causes lymphoproliferation and autoantibody production (3). CD45 variants have been associated with altered immune function and autoimmune disorders including multiple sclerosis, systemic lupus erythematosus (SLE), and rheumatoid arthritis (6). Furthermore, altered CD45 expression has been implicated in oncological conditions including chronic lymphatic leukemia, acute lymphatic leukemia, Hodgkin lymphoma, multiple myeloma, and diffuse large B-cell lymphoma (6). Considering its role in autoimmune disorders, immunodeficiency and cancer, CD45 is an ideal therapeutic target (3, 6). The main approaches to control CD45 function is through either selective inhibitors or anti-CD45 antibodies (3).

Alternative names for CD45 includes B220, CD antigen: CD45, CD45 antigen, CD45R, EC 3.1.3.48, GP180, LCA, Leukocyte common antigen, LY5, protein tyrosine phosphatase receptor type c polypeptide, PTPRC, receptor-type tyrosine-protein phosphatase C, T200 Glycoprotein, and T200.

References

1. Trowbridge, I. S., & Thomas, M. L. (1994). CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annual review of immunology. https://doi.org/10.1146/annurev.iy.12.040194.000505

2. Andersen, J. N., Jansen, P. G., Echwald, S. M., Mortensen, O. H., Fukada, T., Del Vecchio, R., Tonks, N. K., & Moller, N. P. (2004). A genomic perspective on protein tyrosine phosphatases: gene structure, pseudogenes, and genetic disease linkage. FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

3. Hermiston, M. L., Xu, Z., & Weiss, A. (2003). CD45: a critical regulator of signaling thresholds in immune cells. Annual review of immunology. https://doi.org/10.1146/annurev.immunol.21.120601.140946

4. Tonks, N. K., Diltz, C. D., & Fischer, E. H. (1990). CD45, an integral membrane protein tyrosine phosphatase. Characterization of enzyme activity. The Journal of biological chemistry.

5. Nam, H. J., Poy, F., Saito, H., & Frederick, C. A. (2005). Structural basis for the function and regulation of the receptor protein tyrosine phosphatase CD45. The Journal of experimental medicine. https://doi.org/10.1084/jem.20041890

6. Rheinlander, A., Schraven, B., & Bommhardt, U. (2018). CD45 in human physiology and clinical medicine. Immunology letters. https://doi.org/10.1016/j.imlet.2018.01.009

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD45 Antibody (NB100-77417)(48)

Publications using NB100-77417

• Andreas Frøslev Mathisen, Ulrik Larsen, Natalie Kavli, Lucas Unger, Laura Maria Daian, Andrei Mircea Vacaru, Ana-Maria Vacaru, Pedro Luis Herrera, Luiza Ghila, Simona Chera Moderate beta-cell ablation triggers synergic compensatory mechanisms even in the absence of overt metabolic disruption Communications Biology 2024-07-09 [PMID: 38982170]
• Ndjim M, Gasmi I, Herbert F et Al. Tuft cell acetylcholine is released into the gut lumen to promote anti-helminth immunity Immunity 2024-06-12 [PMID: 38744292]
• Yang M, Ong J, Meng F et al. Spatiotemporal insight into early pregnancy governed by immune-featured stromal cells Cell 2023-09-07 [PMID: 37699390]
• Klak M, Wszo?a M, Berman A et al. Bioprinted 3D Bionic Scaffolds with Pancreatic Islets as a New Therapy for Type 1 Diabetes-Analysis of the Results of Preclinical Studies on a Mouse Model Journal of functional biomaterials 2023-07-14 [PMID: 37504866] (IHC, Mouse)
• Wei X, Wang L, Duan C et al. Cardiac patches made of brown adipose-derived stem cell sheets and conductive electrospun nanofibers restore infarcted heart for ischemic myocardial infarction Bioactive materials 2023-09-01 [PMID: 37122901] (In vivo assay, Mouse)
• Reches G, Blondheim Shraga NR, Carrette F et al. Resolving the conflicts around Par2 opposing roles in regeneration by comparing immune-mediated and toxic-induced injuries Inflammation and regeneration 2022-11-29 [PMID: 36447218] (IHC-P, Mouse)
• Wu M, Yu Z, Matar DY et al. Human Amniotic Membrane Promotes Angiogenesis in an Oxidative Stress Chronic Diabetic Murine Wound Model Advances in wound care 2022-03-16 [PMID: 35293255] (IF/IHC, Mouse)
• Yusuf AM, Qaisar R, Al-Tamimi AO Et al. Cardiomyocyte-GSK-3beta deficiency induces cardiac progenitor cell proliferation in the ischemic heart through paracrine mechanisms Journal of cellular physiology 2021-11-23 [PMID: 34812500]
• George KK, Heithoff BP, Shandra O, Robel S Mild traumatic brain injury/ concussion initiates an atypical astrocyte response caused by blood-brain barrier dysfunction Journal of neurotrauma 2021-11-22 [PMID: 34806422]
• Everton E, Rizvi F, Smith AR Et al. Transient yet Robust Expression of Proteins in the Mouse Liver via Intravenous Injection of Lipid Nanoparticle-encapsulated Nucleoside-modified mRNA Bio-protocol 2021-10-05 [PMID: 34722830] (IHC-Fr)
• Wen S, Deng F, Li L et al. VX-765 ameliorates renal injury and fibrosis in diabetes by regulating caspase-1-mediated pyroptosis and inflammation Journal of diabetes investigation 2021-09-08 [PMID: 34494385] (IHC-P, WB, Human, Mouse)
• Wang J, Kural M, Wu J et al. An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies Biomaterials 2021-08-01 [PMID: 34087584]
• Jelinkova S, Sleiman Y, FojtIk P et al. Dystrophin Deficiency Causes Progressive Depletion of Cardiovascular Progenitor Cells in the Heart International Journal of Molecular Sciences 2021-05-10 [PMID: 34068508] (IF/IHC, Mouse)
• Gangoso E, Southgate B, Bradley L et al. Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion Cell 2021-04-29 [PMID: 33857425] (IF/IHC, Mouse)
• Rizvi F, Everton E, Smith AR et Al. Murine liver repair via transient activation of regenerative pathways in hepatocytes using lipid nanoparticle-complexed nucleoside-modified mRNA Nat Commun 2021-01-27 [PMID: 33504774]
• Moiseenko A, Vazquez-Armendariz AI, Kheirollahi V et al. Identification of a Repair-Supportive Mesenchymal Cell Population during Airway Epithelial Regeneration Cell reports 2020-12-22 [PMID: 33357434] (IHC-Fr, Mouse)
• Chen BY Image-guided therapeutic intervention in autoimmune diseases Thesis 2022-01-01 (IF/IHC, Mouse)
• Wutschka J, Kast B, Sator-Schmitt M Et al. JUNB suppresses distant metastasis by influencing the initial metastatic stage Clin Exp Metastasis 2021-07-20 [PMID: 34282521] (WB, Mouse)
• Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (FLOW, Mouse)
  
  Details:
  Citation using the PE format of this antibody.
• Jakubowska MA, Pyka J, Michalczyk-Wetula D et al. Electron paramagnetic resonance spectroscopy reveals alterations in the redox state of endogenous copper and iron complexes in photodynamic stress-induced ischemic mouse liver Redox Biol 2020-05-12 [PMID: 32464500] (IF/IHC, Mouse)
• Simanjuntak Y, Ko HY, Lee YL et al. Preventive effects of folic acid on Zika virus-associated poor pregnancy outcomes in immunocompromised mice PLoS Pathog. 2020-05-01 [PMID: 32392268] (IF/IHC, Mouse)
• Li B, Luan S, Chen J et al. The MSC-Derived Exosomal lncRNA H19 Promotes Wound Healing in Diabetic Foot Ulcers by Upregulating PTEN via MicroRNA-152-3p Molecular Therapy - Nucleic Acids 2020-03-06 [PMID: 31958697] (Flow Cytometry)
• Pei G, Yang L, Li J Et al. Sensory nerves directly promote osteoclastogenesis by secreting Cyp40 Research Square 2021-11-23 (IF/IHC, Rat)
• Chen BY, Ghezzi C, Villegas B et al. 18F-FAC PET visualizes brain-infiltrating leukocytes in a mouse model of multiple sclerosis J. Nucl. Med. 2019-10-25 [PMID: 31653711] (IF/IHC)
• Chang CS, Liao CC, Liou AT et al. Enterovirus 71 targets the cardiopulmonary system in a robust oral infection mouse model Sci Rep 2019-07-31 [PMID: 31366973] (IF/IHC, Mouse)
• Sharifpanah F, Ali EH, Wartenberg M, Sauer H. The milk thistle (Silybum marianum) compound Silibinin stimulates leukopoiesis from mouse embryonic stem cells Phytother Res 2018-12-11 [PMID: 30548344] (WB, Mouse)
• Braun RK, Chetty C, Balasubramaniam V, et al Intraperitoneal injection of MSC-derived exosomes prevent experimental bronchopulmonary dysplasia. Biochem Biophys Res Commun. 2018 Aug 07 [PMID: 30093115] (Flow, Rat)
• Kramann R, Machado F, Wu H et al. Parabiosis and single-cell RNA sequencing reveal a limited contribution of monocytes to myofibroblasts in kidney fibrosis. JCI Insight 2018-05-03 [PMID: 29720573]
• Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018-01-24 [PMID: 29367423] (FLOW, Mouse)
  
  Details:
  This citation used the Alexa Fluor 488 form of this antbody
• Caballero S, Kent DL, Sengupta N et al. Bone Marrow-Derived Cell Recruitment to the Neurosensory Retina and Retinal Pigment Epithelial Cell Layer Following Subthreshold Retinal Phototherapy. Invest. Ophthalmol. Vis. Sci. 2017-10-01 [PMID: 29049716] (IF/IHC, Mouse)
• Khoriaty R, Vogel N, Hoenerhoff MJ et al. SEC23B is required for pancreatic acinar cell function in adult mice. Mol. Biol. Cell 2017-05-24 [PMID: 28539403] (IF/IHC, Mouse)
• Ambrosi TH, Scialdone A, Graja A et al. Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration Cell Stem Cell 2017-03-24 [PMID: 28330582] (FLOW, Mouse)
• Mendes VI, Bartholomeusz GA, Ayres M et al. Synthesis and cytotoxic activity of novel A-ring cleaved ursolic acid derivatives in human non-small cell lung cancer cells Eur J Med Chem 2016-07-22 [PMID: 27484517] (IF/IHC, Mouse)
• Celik Mo, Labuz D, Henning K et al. Leukocyte opioid receptors mediate analgesia via Ca(2+)-regulated release of opioid peptides. Brain Behav. Immun. 2016-04-30 [PMID: 27139929] (ICC/IF, Mouse)
• De Ponti A, Wiechert L, Stojanovic A et al. Chronic liver inflammation and hepatocellular carcinogenesis are independent of S100A9. Int. J. Cancer. 2014-10-21 [PMID: 25331529] (IHC-P, Mouse)
• Tsuboi S, Fukuda M. Overexpression of branched O-linked oligosaccharides on T cell surface glycoproteins impairs humoral immune responses in transgenic mice.. J Biol Chem. 1998-11-13 [PMID: 9804842] (WB)
• Keller G, Wall C, Fong AZ, Hawley TS et al. Overexpression of HOX11 leads to the immortalization of embryonic precursors with both primitive and definitive hematopoietic potential.. Blood. 1998-08-01 [PMID: 9680355] (FLOW)
• Maroun CR, Julius M. Distinct involvement of CD45 in antigen receptor signalling in CD4+ and CD8+ primary T cells.. Eur J Immunol. 1994-04-01 [PMID: 7908636]
• Seaman WE, Talal N, Herzenberg LA et al. Surface antigens on mouse natural killer cells: use of monoclonal antibodies to inhibit or to enrich cytotoxic activity.. J Immunol. 1981-09-01 [PMID: 6790624]
• Raveche ES, Chused TM, Steinberg AD et al. Comparison of response to stem cell differentiation signals between normal and autoimmune mouse strains.. J Immunol. 1985-02-01 [PMID: 3880791] (FLOW, Mouse)
• Pei G, Yang L, Liu B et al. Sensory nerves directly promote osteoclastogenesis by secreting Peptidyl-prolyl cis-trans isomerase D (Cyp40) Research Square 2023-05-08 (IHC-Fr, Mouse)
  
  Details:
  IHC-Fr 1:100
• Condiff EK The Role of Laminin in a Model of Maternal Antiviral Immune Activation During Early Placental Development Thesis 2023-01-01
• Hanaoka K, Fujita N, Lee SH et al. Involvement of CD45 in adhesion and suppression of apoptosis of mouse malignant T-lymphoma cells.. Cancer Res. 1995-05-15 [PMID: 7743522] (Func, Mouse)
  
  Details:
  CD45 antibody (clone 30-F11) was used for studying the effects of anti-CD45 antibody in vitro on murine CS-21 lymphoma cells. The parameters studied were adhesion (Fig. 6) and apoptosis (Fig. 7).
• Ralph SJ, Berridge MV et al. Expression of antigens of the 'T200' family of glycoproteins on hemopoietic stem cells: evidence that thymocyte cell lineage antigens are represented on 'T200'.. J Immunol. 1984-05-01 [PMID: 6425407] (Func, Mouse)
• Seaman WE, Wofsy D, Greenspan JS, Ledbetter JA. Treatment of autoimmune MRL/Ipr mice with monoclonal antibody to Thy-1.2: a single injection has sustained effects on lymphoproliferation and renal disease.. J Immunol. 1983-04-01 [PMID: 6187819] (In Vivo, Mouse)
• Gutstein NL, Seaman WE, Scott JH et al. Induction of immune tolerance by administration of monoclonal antibody to L3T4.. J Immunol. 1986-08-15 [PMID: 3090139] (In Vivo, Mouse)
• Hayakawa K, Carmack CE, Hyman R, Hardy RR. Natural autoantibodies to thymocytes: origin, VH genes, fine specificities, and the role of Thy-1 glycoprotein.. J Exp Med. 1990-09-01 [PMID: 1974916] (In vitro, Mouse)
• Matthews DC, Badger CC, Fisher DR et al. Selective radiation of hematolymphoid tissue delivered by anti-CD45 antibody.. Cancer Res. 1992-03-01 [PMID: 1531324] (In Vivo, Mouse)

Reviews for CD45 Antibody (NB100-77417) (5)

Average Rating: 4.8

(Based on 5 reviews)

We have 5 reviews tested in 2 species: Mouse, Rat.

reviewed by: Verified Customer IHC-P Mouse 10/13/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: glomeruli
• Species: Mouse
• Lot: 30-F11

Comments

• Comments: 4% formaldehyde fixed paraffin sections. EDTA was used for antigen retrieval. 1ry antibody dilution 1:100, 2ry antibody 1:400

reviewed by: Verified Customer IHC-P Mouse 09/15/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse spleen cells
• Species: Mouse
• Lot: B-13

Comments

• Comments: No permeabilization
  Antigen retrieval: Citrate buffer, 20 minutes, 90C
  1st ab dilution: 1:100

reviewed by: Verified Customer IF Rat 11/24/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse Spleen
• Species: Rat

reviewed by: Verified Customer IHC Mouse 10/02/2014

Summary

• Application: Immunohistochemistry
• Sample Tested: mouse liver
• Species: Mouse

reviewed by: Aurora De Ponti IF Mouse 09/05/2014

Summary

• Application: Immunofluorescence
• Sample Tested: mouse liver
• Species: Mouse
• Lot: B144981

FAQs for CD45 Antibody (NB100-77417). (Showing 1 - 4 of 4 FAQs).

1. We would like to order a CD45 antibody to stain immune cells that were isolated from a ligated sciatic nerve of mice with double immunofluorescence (using PFA-fixed tissue on slides). Which of the following rat monoclonal CD45 antibodies would you recommend: 30-F11, IBL-3/16 or 5C16?  ..show answer..
   • I would recommend clone 30-F11. As clone IBL-3/16 has not yet been validated for IHC-P application, we would not be able to guarantee its workability on PFA-fixed tissue. Although both other clones i.e. 30-F11 and 5C16, would be good for your samples, CD45 30-F11 # NB100-77417 is a well known clone that offers more flexibility around protocol because of availability of its conjugated forms. It would be advantageous to use a conjugated primary as you are planning for double-immunostaining procedure.
2. For use in Western Blot with CD45 antibodies, what molecular weight of the band should I expect to see?  ..show answer..
   • The theoretical molecular weight for most of our CD45 antibodies is 147 kDa based off the first isoform. Any variation on 147 is due to the immunogen being from a different species and the protein being a slightly different size. CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated.
3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?  ..show answer..
   • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product, allowing the tested application/species to fall under our 100% guarantee. Please check out our Innovator's Reward Program if you decide to test a primary antibody with a species or application that is not currently listed. Please note that the Innovator's Reward Program only applies to our primary antibodies.
4. Is CD45 a good target for use in Neurodegeneration studies?  ..show answer..
   • Yes, here are the approved research areas we have listed for our CD45 products: Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Glia Markers, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Microglia Markers, Myeloid Cell Markers, Myeloid-derived Suppressor, Neurodegeneration, Neuroscience, Signal Transduction, Stem Cell Markers, Growth and Development.

Bioinformatics

• Gene Symbol: PTPRC
• Entrez:
  • 19264(Mouse)
• Uniprot:
  • P06800(Mouse)