CD58/LFA-3 Antibody (2D11-B10) - Azide and BSA Free

Western Blot: CD58/LFA-3 Antibody (2D11-B10) [H00000965-M01] - CD58 monoclonal antibody (M01), clone 2D11-B10 Analysis of CD58 expression in Jurkat.

Immunocytochemistry/Immunofluorescence: CD58/LFA-3 Antibody (2D11-B10) [H00000965-M01] - Analysis of monoclonal antibody to CD58 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (2D11-B10) [H00000965-M01] - Analysis of monoclonal antibody to CD58 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 2 ug/ml.

Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (2D11-B10) [H00000965-M01] - Analysis of monoclonal antibody to CD58 on formalin-fixed paraffin-embedded human lymphoma tissue Antibody concentration 3 ug/ml.

ELISA: CD58/LFA-3 Antibody (2D11-B10) [H00000965-M01] - Detection limit for recombinant GST tagged CD58 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2D11-B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CD58/LFA-3 Antibody (2D11-B10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CD58 (AAH05930, 1 a.a. ~ 240 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVAGSDAGRALGVLSVVCLLHCFGFISCFSQQIYGVVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSEFRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEMESPNITDTMKFFLYVLESLPSPTLTCALTNGSIEVQCMIPEYYNSHRGLIMYSWDCPMEQCKRNSTSIYFKMENDLPQKIQCTLSNPLFNTTSSIILTTCIPSSGHSRHRYALIPIPLAVITTCIVLYMNGMYAF
• Localization: Type I membrane protein (isoform 1) and attached to the membrane by a GPI-anchor (isoform 2).
• Specificity: CD58 - CD58 antigen, (lymphocyte function-associated antigen 3)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD58
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CD58/LFA-3 Antibody (2D11-B10) - Azide and BSA Free

• Ag3
• CD58 antigen
• CD58 antigen, (lymphocyte function-associated antigen 3)
• CD58 molecule
• CD58
• FLJ23181
• FLJ43722
• LFA3
• LFA-3
• LFA3ag3
• lymphocyte function-associated antigen 3
• Surface glycoprotein LFA-3

Background

LFA3 is a type 1 transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed on most hematopoietic cells and on nonhematopoietic cells such as fibroblasts, endothelial and epithelial cells. Its expression is particularly high on memory T-cells and dendritic cells. LFA3 expression on antigen-presenting cells and target cells enhances T-cells antigen recognition through binding to its ligand, LFA2/CD2, on T-cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD58
• Entrez:
  • 965(Human)
• OMIM:
  • 153420(Human)
• Uniprot:
  • AAH05930(Human)