CD7 Antibody (848438) [Unconjugated]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A) and either (A) Mouse Anti-Human CD7 Monoclonal Antibody (Catalog # MAB7579) or (B) Mouse IgG2BFlow Cytometry Isotype Control (MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 848438
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD7 Antibody (848438) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived human CD7
  Ala26-Pro180
  Accession # P09564
• Specificity: Detects human CD7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD7 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD7 Antibody (848438) [Unconjugated]

• CD7 antigen (p41)
• CD7 antigen
• CD7 molecule
• CD7
• GP40T-cell surface antigen Leu-9
• LEU-9
• T-cell antigen CD7
• T-cell leukemia antigen
• Tp40
• TP41
• TP41p41 protein

Background

CD7 (Cluster of Differentiation Antigen 7; also Leu-9, TP41 and GP40) is a 40-44 kDa member of the Ig-superfamily of proteins. It shows restricted expression, being found on fetal thymocytes, CD34⁺ myeloid and lymphoid progenitor cells, memory CLA- CD45RA⁺ T cells, and CD56⁺ IFN-gamma secreting NK cells. CD7 binds to both SECTM1/K12 and galectin-1, and when bound to the latter, initiates complex formation with CD43 in cis. Activation of CD7 may result in either cell proliferation or apoptosis, suggesting a context-dependent signaling mechanism. Mature human CD7 is a 215 amino acid (aa) type I transmembrane glycoprotein. It contains a 155 aa extracellular region (aa 26-180) that shows one V-type Ig-like domain (aa 26-130), and a 39 aa C-terminal cytoplasmic domain. There is one potential alternative splice variant that contains a 79 aa substitution for aa 133-240. Over aa 26-180, human CD7 shares only 43% aa sequence identity with mouse CD7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD7
• Uniprot:
  • P09564(Human)