CD74 Antibody (CerCLIP.1) - BSA Free

Flow Cytometry: CD74 Antibody (CerCLIP.1) [NBP1-19249] - A cell surface stain was performed on Daudi cells with CD74 Antibody (CerCLIP.1) NBP1-19249AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

• Reactivity: Hu
• Applications: Flow, IP
• Clone: CerCLIP.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

CD74 Antibody (CerCLIP.1) - BSA Free Summary

• Immunogen: Synthetic peptide derived from the sequence of human CLIP
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD74
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:200
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for CD74 Antibody (CerCLIP.1) - BSA Free

• CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated)
• CD74 antigen
• CD74 molecule, major histocompatibility complex, class II invariant chain
• CD74
• CLIP
• DHLAG
• DHLAGgamma chain of class II antigens
• HLA class II histocompatibility antigen gamma chain
• HLADG
• HLA-DR antigens-associated invariant chain
• HLA-DR-gamma
• Ia antigen-associated invariant chain
• Ia-associated invariant chain
• Ia-gamma
• Ii
• INVG34
• MHC HLA-DR gamma chain
• p33

Background

CLIP (class II-associated invariant chain peptide) is an important peptide in the MHC-II antigen processing pathway, formed by the enzymatic cleavage of amino acids 81-104 from the invariant chain. During MHC-II synthesis in the ER and transport to endocytic vesicles, the antigen binding site of MHC-II is obstructed by the presence of the invariant chain. As the MHC-II complex moves through the Golgi, the invariant chain is degraded, leaving the CLIP fragment in the MHC-II peptide binding groove. CLIP is eventually displaced by the loading of antigenic peptides, followed by presentation on the APC cell surface to CD4+ T-cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD74 Antibody (NBP1-19249)(7)

Publications using NBP1-19249

• Ghahremani M, Habil MK, Zapata-Rodriguez CJ. Anapole-assisted giant electric field enhancement for surface-enhanced coherent anti-Stokes Raman spectroscopy. Scientific reports 2021-05-20 [PMID: 34017020]
• Jiang W, Adler LN, Macmillan H, Mellins ED et al. Synergy between B cell receptor/antigen uptake and MHCII peptide editing relies on HLA-DO tuning Sci Rep. 2019-09-25 [PMID: 31554902] (FLOW, Human)
  
  Details:
  Citation used the Alexa Fluor 647 format of this antibody.
• Ladowski JM, Martens GR, Reyes LM et al. Examining epitope mutagenesis as a strategy to reduce and eliminate human antibody binding to class II swine leukocyte antigens Immunogenetics 2019-07-01 [PMID: 31270568] (FLOW)
• Ladowski JM, Martens GR, Reyes LM et al. Examining the Biosynthesis and Xenoantigenicity of Class II Swine Leukocyte Antigen Proteins. J. Immunol. 2018-03-14 [PMID: 29540579] (FLOW)
• Denzin LK, Hammond C, Cresswell P et al. HLA-DM interactions with intermediates in HLA-DR maturation and a role for HLA-DM in stabilizing empty HLA-DR molecules. J Exp Med. 1996-12-01 [PMID: 8976171]
• Denzin LK, Cresswell P. HLA-DM induces CLIP dissociation from MHC class II alpha beta dimers and facilitates peptide loading. Cell. 1995-07-01 [PMID: 7606781]
• Denzin LK, Robbins NF, Carboy-Newcomb C et al. Assembly and intracellular transport of HLA-DM and correction of the class II antigen-processing defect in T2 cells. Immunity. 1994-10-01 [PMID: 7600288]

Bioinformatics

• Gene Symbol: CD74
• Entrez:
  • 972(Human)