CD9 Antibody (IVA50) - BSA Free

Immunoprecipitation: CD9 Antibody (IVA50) [NB500-494] - Fig. 1. Immunoprecipitation of human CD9 from the biotin-labeled human platelets lysates. Lane 1: immunoprecipitation with anti-CD9 (IVA50) Lane 2: immunoprecipitation with anti-human CD9 control antibody Lane 3: immunoprecipitation with negative control.

Separation of bovine CD9 positive thrombocytes (red-filled) from lymphocytes (black-dashed) in Analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD9 (IVA50) purified antibody (concentration in sample 10 ug/ml) GAM APC.

• Reactivity: Hu, Bv
• Applications: WB, Flow, IP, CyTOF-ready
• Clone: IVA50
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD9 Antibody (IVA50) - BSA Free Summary

• Immunogen: Bovine thrombocytes.
• Specificity: The antibody IVA50 reacts with CD9 antigen (bovine), a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:50
  • Western Blot 1 ug/ml
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD9 Antibody (IVA50) - BSA Free

• BA2
• BA-2/p24 antigen
• BTCC-1
• CD9 antigen
• CD9 molecule
• CD9
• Cell growth-inhibiting gene 2 protein
• DRAP-27
• Leukocyte antigen MIC3
• MIC3
• motility related protein-1,5H9 antigen
• Motility-related protein
• MRP-1FLJ99568
• p24
• tetraspanin-29
• TSPAN29
• TSPAN-29
• TSPAN29MIC3CD9 antigen (p24)

Background

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD9 Antibody (NB500-494)(8)

Publications using NB500-494

• Han, G;Kim, H;Jang, H;Kim, ES;Kim, SH;Yang, Y; Oral TNF-? siRNA delivery via milk-derived exosomes for effective treatment of inflammatory bowel disease Bioactive materials 2024-04-01 [PMID: 38223538]
• Turner N, Abeysinghe P, Flay H et al. SWATH-MS Analysis of Blood Plasma and Circulating Small Extracellular Vesicles Enables Detection of Putative Protein Biomarkers of Fertility in Young and Aged Dairy Cows Journal of proteome research 2023-11-03 [PMID: 37830897]
• Turner NP, Abeysinghe P, Sadowski P, Mitchell MD Omics Analysis of Extracellular Vesicles Recovered from Infant Formula Products and Milk: Towards Personalized Infant Nutrition Molecular nutrition & food research 2023-08-10 [PMID: 37562982] (Western Blot, Bovine, Human)
• Liu W, Du C, Nan L et al. Influence of Estrus on Dairy Cow Milk Exosomal miRNAs and Their Role in Hormone Secretion by Granulosa Cells International journal of molecular sciences 2023-06-01 [PMID: 37298559]
• Go G, Park HJ, Lee JH et al. Inhibitory Effect of Oxaliplatin-loaded Engineered Milk Extracellular Vesicles on Tumor Progression Anticancer research 2022-02-01 [PMID: 35093883]
• Marsh S, Pridham K, Jourdan J, Gourdie R Novel Protocols for Scalable Production of High Quality Purified Small Extracellular Vesicles from Bovine Milk Nanotheranostics 2021-07-27 [PMID: 34367882] (WB)
• Go G, Jeon J, Lee G et al. Bovine milk extracellular vesicles induce the proliferation and differentiation of osteoblasts and promote osteogenesis in rats. J Food Biochem 4427-01-01 [PMID: 33748986] (WB)
• Rahman MM, Shimizu K, Yamauchi M et al. Acidification effects on isolation of extracellular vesicles from bovine milk PLoS ONE 2019-09-16 [PMID: 31525238] (WB, Bovine)

Bioinformatics

• Gene Symbol: CD9