CD90/Thy1 Antibody (G7) [Biotin]

Flow Cytometry: CD90/Thy1 Antibody (G7) [Biotin] [NBP1-28033] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD90-BIOT and Rat Anti-Mouse CD3 epsilon-PE followed by Streptavidin-FITC.

• Reactivity: Mu
• Applications: Flow, Func, ICC/IF, IHC, IP
• Clone: G7
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: 0.5 mg/ml

CD90/Thy1 Antibody (G7) [Biotin] Summary

• Immunogen: Mouse T cell hybridoma C6/G8
• Marker: Mesenchymal Cell Marker
• Specificity: Mouse CD90 (Thy-1.1 & Thy-1.2 alloantigens/Thy-1 epitope region A); Mr 28-32 kDa
• Isotype: IgG2c Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: THY1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Notes

Conjugated forms should be protected from prolonged exposure to light.

Alternate Names for CD90/Thy1 Antibody (G7) [Biotin]

• CD90 antigen
• CD90
• CDw90
• FLJ33325
• Thy-1 antigen
• Thy-1 cell surface antigen
• thy-1 membrane glycoprotein
• Thy-1 T-cell antigen
• Thy1

Background

CD90/Thy-1, a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily of cell surface receptors, consists of a single V-set domain. (1-4) It is expressed on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes and neurons of all mouse strains. (1,2) MAb G7 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex. (1,5-7) The MAb has also been reported to promote apoptosis of thymocytes and CTL clones,4,7 and to mediate adhesion of thymocytes to thymic stroma.8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD90/Thy1 Antibody (NBP1-28033)(6)

Publications using NBP1-28033

• Matsumura M, Watanabe YY, Robinson PW et al. Underwater and surface behavior of homing juvenile northern elephant seals. The Journal of experimental biology 2011-06-02 [PMID: 21270312]
• Hueber, AO, G Raposo, M Pierres, HT He. Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism J Exp Med 1994-03-01 [PMID: 7906706]
  
  Details:
  This citation used the Biotin version of this antibody.
• Gunter, K, T Malek, E Shevach. T cell-activating properties of an anti-Thy-1 monoclonal antibody, possible analogy to OKT3/Leu-4 J Exp Med 1984-03-01 [PMID: 6142077] (In vitro)
  
  Details:
  This citation used the Biotin version of this antibody.
• Tentori, L, DM Pardoll, JC Zunigaq et al. Proliferation and production of IL-2 and B cell stimulatory factor 1/IL-4 in early fetal thymocytes by activation through Thy-1 and CD3 J Immunol 1988-02-01 [PMID: 2893823] (In vitro)
  
  Details:
  This citation used the Biotin version of this antibody.
• Sugiyama, E, A Cantagrel, T Reno et al. Selection of T cell receptor expression mutants through the functionally linked Ly-6A Cell Immunol 1990-10-01 [PMID: 1976441] (In vitro)
  
  Details:
  This citation used the Biotin version of this antibody.
• Ucker, DS, J Meyers, PS Obermiller. Activation-driven T cell death II Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death J Immunol 1992-09-01 [PMID: 1380534]
  
  Details:
  This citation used the Biotin version of this antibody.

FAQs for CD90/Thy1 Antibody (NBP1-28033). (Showing 1 - 1 of 1 FAQs).

1. We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.

Bioinformatics

• Gene Symbol: THY1