CD90/Thy1 Antibody (OX-7) - BSA Free

Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus.

Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus with MOUSE ANTI RAT CD90 (THY 1.1).

Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymocytes.

Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymocytes.

Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus cell.

• Reactivity: Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP, IHC
• Clone: OX-7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CD90/Thy1 Antibody (OX-7) - BSA Free Summary

• Immunogen: Rat Thy 1 antigen
• Marker: Mesenchymal Cell Marker
• Specificity: This antibody is specific for CD90.1
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: THY1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry Free-Floating reported in scientific literature (PMID 35383427)
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:28928774).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for CD90/Thy1 Antibody (OX-7) - BSA Free

• CD90 antigen
• CD90
• CDw90
• FLJ33325
• Thy-1 antigen
• Thy-1 cell surface antigen
• thy-1 membrane glycoprotein
• Thy-1 T-cell antigen
• Thy1

Background

CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD90/Thy1 Antibody (NB100-65543)(19)

Publications using NB100-65543

• Weng Y, Feng Y, Li Z et Al. Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells Nat Commun 2024-11-24 [PMID: 39582024]
• Wei X, Wang L, Duan C et al. Cardiac patches made of brown adipose-derived stem cell sheets and conductive electrospun nanofibers restore infarcted heart for ischemic myocardial infarction Bioactive materials 2023-09-01 [PMID: 37122901] (ICC/IF, Rat)
• Bai L, Liu Y, Zhang X et al. Osteoporosis remission via an anti-inflammaging effect by icariin activated autophagy Biomaterials 2023-04-10 [PMID: 37058900] (IHC-P, Mouse)
• Consalvi S, Tucciarone L, MacrI E et al. Determinants of epigenetic resistance to HDAC inhibitors in dystrophic fibro-adipogenic progenitors EMBO reports 2022-04-04 [PMID: 35383427] (IHC-FrFl, Mouse)
• Zhang N, Luo Y, Zhang H et al. Exosomes Derived from Mesenchymal Stem Cells Ameliorate the Progression of Atherosclerosis in ApoE-/- Mice via FENDRR Cardiovascular toxicology [PMID: 35344140]
• Liu J, Zhou L, Zhao F et al. Therapeutic effect of adipose stromal vascular fraction spheroids for partial bladder outlet obstruction induced underactive bladder Stem cell research & therapy 2022-02-09 [PMID: 35139904] (FLOW, Rat)
  
  Details:
  Adipose tissue around epididymis was collected and ad-SVF were isolated; conjugate with Allophycocyanin (APC)
• Tigges J, Eggerbauer F, Worek F Et al. Optimization of long-term cold storage of rat precision cut lung slices (PCLS) with a tissue preservation solution American journal of physiology. Lung cellular and molecular physiology 2021-10-13 [PMID: 34643087] (FLOW, Rat)
• Zhang J, Wang JH. Maintenance of Tendon Stem/Progenitor Cells in Culture. Methods Mol. Biol. 2018-09-10 [PMID: 30196413] (ICC/IF, Rat)
• Hassanzadeh H, Matin MM, Naderi-Meshkin H et al. Using paracrine effects of Ad-MSCs on keratinocyte cultivation and fabrication of epidermal sheets for improving clinical applications Cell Tissue Bank 2018-08-13 [PMID: 30105667] (FLOW)
• Li F, Ma Z et al. Y-box Protein-1 Regulates the Expression of Collagen I in Hepatic Progenitor Cells via PDGFR-beta/ERK/p90RSK Signalling. Stem Cells Int 2017-09-21 [PMID: 28928774] (IF/IHC, Mouse)
• Hoseini SJ, Ghazavi H, Forouzanfar F et al. Fibroblast Growth Factor 1-Transfected Adipose-Derived Mesenchymal Stem Cells Promote Angiogenic Proliferation. DNA Cell Biol. 2017-03-10 [PMID: 28281780]
• Homayounfar N, Verma P, Nosrat A et al. Isolation, Characterization, and Differentiation of Dental Pulp Stem Cells in Ferrets. J Endod. 2016-01-12 [PMID: 26794343] (ICC/IF)
• Biermann J, Boyle J, Pielen A et al. Histone deacetylase inhibitors sodium butyrate and valproic acid delay spontaneous cell death in purified rat retinal ganglion cells. Mol Vis. 2011-02-01 [PMID: 21311741]
• Ohashi N, Urushihara M, Satou R et al. Glomerular angiotensinogen is induced in mesangial cells in diabetic rats via reactive oxygen species--ERK/JNK pathways. Hypertens Res. 2010-11-01 [PMID: 20686488]
• Stevenson KS, McGlynn L, Hodge M et al. Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations. Stem Cells Dev. 2009-12-01 [PMID: 19326970]
• Khaldoyanidi S, Schnabel D, Fohr N, Zoller M. Functional activity of CD44 isoforms in haemopoiesis of the rat. Br J Haematol. [PMID: 9012685] (FLOW, Rat)
• Crook K, Hunt SV. Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82. Dev Immunol. [PMID: 8924759] (FLOW, Rat)
• Lazo PA, Klein-Szanto AJ, Tsichlis PN. T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications. J Virol. [PMID: 2196385] (FLOW, Rat)
• Brown G Adipose-derived stromal vascular fraction therapy improves age-related adrenergic mediated microvascular dysfunction and increases revascularization potential following injury. Thesis 2022-01-01

FAQs for CD90/Thy1 Antibody (NB100-65543). (Showing 1 - 2 of 2 FAQs).

1. We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.
2. Can I use Cd90/Thy1antibody (OX-7) NB100-65543 for mouse tissue to do Western Blot? What additional optimization is required?
   • Our datasheet says that this antibody can be used in these applications: WB, Flow, IHC-Fr, and IP with the samples from mouse, rat, guinea pig, and rabbit. If using this antibody in above applications with the sample(s) from the species, we guarantee it will produce the positive results, or we will refund or replace the antibody free. [When using in IHC-Fr, the mouse-on-mouse (http://vectorlabs.com/mouse-on-mouse-m-o-m-blocking-reagent.html) will be required]. It does not have any special instructions for using this antibody in WB, and therefore our general WB protocol can apply: Protocol. Our recommendation for dilution in WB: 1:100-1:2000. Use the above protocol/dilution to start a small test first. After getting the positive result(s), you could adjust the dilution factor of the antibody as you are repeating/duplicating the experiment, to obtain the best image(s).

Bioinformatics

• Gene Symbol: THY1
• Entrez:
  • 21838(Mouse)
  • 100339279(Rabbit)
  • 24832(Rat)
• Uniprot:
  • P01830(Rat)