CD90.2 Antibody (30-H12) - Azide and BSA Free

Flow Cytometry: CD90.2 Antibody (30-H12) [NBP1-28036] - Analysis using the Allophycocyanin conjugate of NBP1-28036. Multiple staining of BALB/c splenocytes.

• Reactivity: Mu
• Applications: Flow
• Clone: 30-H12
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

CD90.2 Antibody (30-H12) - Azide and BSA Free Summary

• Immunogen: Mouse thymus or spleen
• Specificity: Mouse CD90.2/Thy-1.2 alloantigen, Mr 18 kDa
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: THY1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD90.2 Antibody (30-H12) - Azide and BSA Free

• CD90 antigen
• CD90
• CDw90
• FLJ33325
• Thy-1 antigen
• Thy-1 cell surface antigen
• thy-1 membrane glycoprotein
• Thy-1 T-cell antigen

Background

CD90/Thy-1 is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily, consisting of a single V-set domain. (1-3) The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues. The 30-H12 monoclonal antibody does not cross react with mouse and rat strains bearing the Thy-1.1 alloantigen (e.g., AKR/J, PL). (2-5)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD90.2 Antibody (NBP1-28036)(3)

Publications using NBP1-28036

• Anthony SM, Van Braeckel-Budimir N, Moioffer SJ, van de Wall S et Al. Protective function and durability of mouse lymph node-resident memory CD8(+) T cells Elife 2021-06-18 [PMID: 34143731]
• Alberton P, Dex S, Popov C et al. Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells. Stem Cells Dev. 2014-10-28 [PMID: 25351164]
• McLellan AD, Kapp M, Eggert A et al. Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression. Blood. 2002-03-15 [PMID: 11877283] (FLOW, Mouse)
  
  Details:
  This citation used the FITC version of this antibody.

FAQs for CD90.2 Antibody (NBP1-28036). (Showing 1 - 1 of 1 FAQs).

1. I am looking for an anti-mouse Thy1.2 to use in immunofluorescence. However, my samples are formalin-fixed paraffin embedded (FFPE). Have any of your antibodies been tested for reactivity against fixed epitopes that would be worth trying on my samples?
   • We have not tested these antibodies in IHC-P yet, however, I do not see any reason why these products should not work in this application! Because this product worked well in IHC-Fr and FLOW applications wherein the antibodies bind the antigen in relatively native state/sub-cellular form, we strongly believe that it should work in IHC-P also! However, you will certainly need to play around the antigen retrieval timings (depending upon for how long your tissues were kept for fixation in formalin) to get your staining procedure optimized.

Bioinformatics

• Gene Symbol: THY1