CD90.2 Antibody (53-2.1) - BSA Free

Flow Cytometry: CD90.2 Antibody (53-2.1) [NBP1-43380] - Analysis using the FITC conjugate of NBP1-43380. Staining of mouse splenocytes with Anti-Mouse CD90.2 (Thy-1.2) FITC (left ) or PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

• Reactivity: Mu
• Applications: Flow, IHC, IP
• Clone: 53-2.1
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CD90.2 Antibody (53-2.1) - BSA Free Summary

• Immunogen: The immunogen for this antibody was CD90.2.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: THY1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: The 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.06 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD90.2 Antibody (53-2.1) - BSA Free

• CD90 antigen
• CD90
• CDw90
• FLJ33325
• Thy-1 antigen
• Thy-1 cell surface antigen
• thy-1 membrane glycoprotein
• Thy-1 T-cell antigen

Background

The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CD90.2 Antibody (NBP1-43380). (Showing 1 - 1 of 1 FAQ).

1. I am looking for an anti-mouse Thy1.2 to use in immunofluorescence. However, my samples are formalin-fixed paraffin embedded (FFPE). Have any of your antibodies been tested for reactivity against fixed epitopes that would be worth trying on my samples?
   • We have not tested these antibodies in IHC-P yet; however, I do not see any reason why these products should not work in this application! Because this product worked well in IHC-Fr and FLOW applications wherein the antibodies bind the antigen in relatively native state/sub-cellular form, we strongly believe that it should work in IHC-P also! However, you will certainly need to play around the antigen retrieval timings (depending upon for how long your tissues were kept for fixation in formalin) to get your staining procedure optimized.

Bioinformatics

• Gene Symbol: THY1