CD97 Antibody (MEM-180) [FITC]

Flow Cytometry: CD97 Antibody (MEM-180) [FITC] [NBP1-44881] - Surface staining of human peripheral blood with anti-CD97 (MEM-180) FITC.

• Reactivity: Hu
• Applications: Flow, IP
• Clone: MEM-180
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

CD97 Antibody (MEM-180) [FITC] Summary

• Description: Store in the dark. Avoid prolonged exposure to light.
• Additional Information: Clone MEM-180 was used by HLDA to establish CD designation.
• Immunogen: PHA-activated peripheral blood cells
• Localization: Plasma membrane
• Specificity: The antibody MEM-180 recognizes an unique epitope on CD97, a 75-85 kDa surface glycoprotein of G-protein-coupled receptor family, expressed on activated B and T lymphocytes, monocytes/macrophages, dendritic cells and granulocytes. HLDA VI; WS Code BP 415 HLDA VI; WS Code NL N-L023
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADGRE5
• Purity: Size Exclusion Chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoprecipitation 1:10-1:500
• Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS and 0.2% (w/v) high grade BSA (protease free)
• Preservative: 0.09% Sodium Azide
• Purity: Size Exclusion Chromatography

Alternate Names for CD97 Antibody (MEM-180) [FITC]

• CD97 antigenseven-span transmembrane protein
• CD97 molecule
• Leukocyte antigen CD97
• seven transmembrane helix receptor
• seven-transmembrane, heterodimeric receptor associated with inflammation
• TM7LN1

Background

CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADGRE5