CDK4 Antibody (DCS-31)

Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, MA
• Clone: DCS-31
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CDK4 Antibody (DCS-31) Summary

• Immunogen: Recombinant human Cdk4 protein
• Localization: Cytoplasmic (likely)
• Specificity: Reacts specifically with Cdk4. Does not recognize other Cdk types.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDK4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1:1000
• Application Notes: WB: recognizes a 33kD band, and it may pick up additional weaker bands, using a cultured human tumor cell line extract.
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CDK4 Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for CDK4 Antibody (DCS-31)

• CDK4
• Cell division protein kinase 4
• CMM3
• cyclin-dependent kinase 4
• EC 2.7.11
• EC 2.7.11.22
• melanoma cutaneous malignant, 3
• MGC14458
• PSK-J3
• PSK-J3cell division kinase 4

Background

Cdk4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). The mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Two alternatively spliced variants, and multiple polyadenylation sites of this gene have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDK4 Antibody (NB120-6315)(2)

Publications using NB120-6315

• Li K, Huang G, Wang Z et al. IKBIP, a novel glioblastoma biomarker, maintains abnormal proliferation of tumor cells by inhibiting the ubiquitination and degradation of CDK4 Biochimica et biophysica acta. Molecular basis of disease 2022-10-14 [PMID: 36244542]
• Brookes S, Gagrica S, Sanij E et al. Evidence for a CDK4-dependent checkpoint in a conditional model of cellular senescence Cell Cycle. 2015-02-19 [PMID: 25695870] (WB)

Bioinformatics

• Gene Symbol: CDK4
• Entrez:
  • 1019(Human)
  • 12567(Mouse)
  • 94201(Rat)
• Uniprot:
  • P11802()