Cdk5 Antibody (1H3) - Azide Free

Western Blot: Cdk5 Antibody [NBP1-47638] - Rat Caudate Lysate showing specific immunolabeling of the ~28k Cdk5 protein.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 1H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free

Cdk5 Antibody (1H3) - Azide Free Summary

• Immunogen: Purified rat Cdk5. Accession # Q03114
• Specificity: The antibody is specific for the ~ 28kDa Cdk5 protein in Western blots of rat striatal lysate. The antibody shows no cross reactivity with the Cdk5 cofactor p35 or its degradation product p25. This monoclonal also works well for immunocytochemistry using primary cultured rat neurons and for immunohistochemistry on mouse brain tissue.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDK5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:1000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Tested in Human, Mouse and Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Cdk5 Antibody (1H3) - Azide Free

• CDK5
• CDKN5
• Cell division protein kinase 5
• cyclin-dependent kinase 5
• EC 2.7.11
• EC 2.7.11.22
• LIS7
• protein kinase CDK5 splicing
• PSSALRE
• Serine/threonine-protein kinase PSSALRE
• Tau protein kinase II catalytic subunit
• TPKII catalytic subunit

Background

Cyclin-dependent protein kinase 5 (Cdk5) is a unique member of the family of cyclin-dependent kinases that is not directly involved in the progression of proliferating cells through the cell cycle (1). Distributed in most tissues and various cell lines, Cdk5 is active primarily in neuronal tissues, because of its association with neuron-specific activators such as p35 (a neural-specific regulatory subunit of Cdk5) (2). In addition to its role in muscle and neuronal differentiation, Cdk5 also contributes to the assembly, organization and stability of the axonal cytoskeleton in neurons, promotes neurite outgrowth and axon guidance in developing neurons, participates in the regulation of synaptic transmission and provides an additional pathway to cellular apoptosis (3-5). Cdk5 phosphorylates MAP1b, Tau, neurofilament proteins, caldesmon and proteins of the synaptic vesicles. Deregulation of Cdk5 has profound cytotoxic effects and is implicated in the development of neurodegenerative diseases such as Alzheimer's disease and amyotrophic lateral sclerosis (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK5
• Entrez:
  • 140908(Rat)
• Uniprot:
  • Q03114(Rat)