CDX2 Antibody (AMT28)

Immunohistochemistry-Paraffin: CDX2 Antibody (AMT28) [NB100-699] - Human colon.

Immunohistochemistry-Paraffin: CDX2 Antibody (AMT28) [NB100-699] - Formalin fixed paraffin embedded human colon stained with Cdx2 antibody.

Product Discontinued

CDX2 Antibody (AMT28) Summary

• Immunogen: Prokaryotic recombinant protein corresponding to the 180 amino acid N-terminal region of the human CDX2 molecule.
• Localization: Nuclear
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDX2
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:25-1:50
• Application Notes: Immunohistochemistry (paraffin-embedded sections): ABC method with a suggested incubation period of 60 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1 mM EDTA buffer, pH 8.0 prior to immunostaining. Use in ICC/IF was reported in scientific literature (PMID: 31249412).
• Control:

  CDX2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Tissue culture supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for CDX2 Antibody (AMT28)

• caudal type homeo box transcription factor 2
• caudal type homeobox 2
• Caudal-type homeobox protein 2
• CDX2
• CDX3
• CDX-3
• CDX3caudal type homeobox transcription factor 2
• homeobox protein CDX-2

Background

CDX2 is a caudal type homeobox gene that encodes an intestine-specific transcription factor that is expressed early in intestinal development and may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells. It is expressed in the nuclei of epithelial cells throughout the intestine, from duodenum to rectum. The CDX2 protein is expressed in primary and metastatic colorectal carcinomas and has also been demonstrated in the intestinal metaplasia of the stomach and intestinal-type gastric cancer, while it is not expressed in the normal gastric mucosa. Studies have shown that CDX2 is superior marker compared to CK20.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDX2 Antibody (NB100-699)(2)

Publications using NB100-699

• Manfrin A, Tabata Y, Paquet ER et al. Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells Nat. Methods 2019-07-01 [PMID: 31249412] (ICC/IF, Human)
• Oyama Y, Nishida H, Kusaba T et al. Colon adenoma and adenocarcinoma with clear cell components - two case reports Diagn Pathol 2019-05-10 [PMID: 31077226] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: CDX2
• Entrez:
  • 1045(Human)
• Uniprot:
  • Q99626()