CEACAM/CD66 Antibody (COL-1) - IHC-Prediluted

Immunohistochemistry-Paraffin: CEACAM/CD66 Antibody (COL-1) - IHC-Prediluted [NBP2-48038] - Formalin-fixed, paraffin-embedded normal human colon stained with CEA COL-1 antibody at 1:200 using peroxidase-conjugate and DAB chromogen.

Immunohistochemistry-Paraffin: CEACAM/CD66 Antibody (COL-1) - IHC-Prediluted [NBP2-48038] - Staining of colon cancer.

Immunohistochemistry-Paraffin: CEACAM/CD66 Antibody (COL-1) - IHC-Prediluted [NBP2-48038] - Formalin-fixed, paraffin-embedded human colon cancer stained with CEA COL-1 antibody at 1:200 using peroxidase-conjugate and DAB chromogen.

Product Discontinued

CEACAM/CD66 Antibody (COL-1) - IHC-Prediluted Summary

• Immunogen: Colon carcinoma cell extract was used as the immunogen for the CEACAM1 antibody.
• Localization: Cytoplasmic and luminal surface
• Specificity: This antibody recognizes proteins of 80-200kDa, identified as different members of CEACAM family. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEACAM is not found in benign glands, stroma, or malignant prostatic cells
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEACAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Hu-chromosome location: 1q32.2
  Molecular weight of antigen: 90-110kDa
  Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEACAM1