Cerberus 1 Antibody (162836)

Cerberus 1 was detected in immersion fixed paraffin-embedded sections of human liver using 25 µg/mL Mouse Anti-Human Cerberus 1 Monoclonal Antibody (Catalog # MAB1075) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows secondary antibody only control experiment. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 162836
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cerberus 1 Antibody (162836) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cerberus 1
  Thr18-Ala267 (predicted)
  Accession # O95813
• Specificity: Detects human Cerberus 1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) DAN, rhGremlin, or recombinant chicken Caronte is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CER1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cerberus 1 Antibody (162836)

• CER1
• cerberus 1 (Xenopus laevis) homolog (cysteine knot superfamily)
• Cerberus 1
• cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis)
• cerberus
• cerberus-related 1
• Cerberus-related protein
• DAN domain family member 4
• DAND4
• MGC119894
• MGC119895
• MGC96951

Background

Cerberus 1 functions during development by binding to and antagonizing some members of the bone morphogenetic protein (BMP) family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cerberus 1 Antibody (MAB1075)(1)

Publication using MAB1075

• Hidefumi Iwashita, Nobuaki Shiraki, Daisuke Sakano, Takashi Ikegami, Masanobu Shiga, Kazuhiko Kume et al. Secreted Cerberus1 as a Marker for Quantification of Definitive Endoderm Differentiation of the Pluripotent Stem Cells PLoS ONE 2013-05-22 [PMID: 23717584]

Bioinformatics

• Gene Symbol: CER1
• Uniprot:
  • O95813()