CHAC1 Antibody (OTI1E2)

Western Blot: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAC1.

Immunocytochemistry/Immunofluorescence: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CHAC1.

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: CHAC1 Antibody (OTI1E2) [NBP2-46273] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI1E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CHAC1 Antibody (OTI1E2) Summary

• Immunogen: Full length human recombinant protein of human CHAC1(NP_077016) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHAC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:4000
• Theoretical MW: 24.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CHAC1 Antibody (OTI1E2)

• cation transport regulator-like protein 1
• ChaC, cation transport regulator homolog 1 (E. coli)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHAC1 Antibody (NBP2-46273)(1)

Publication using NBP2-46273

• Donovan J, Deng Z, Bian F et al. Improving anti-tumor efficacy of low-dose Vincristine in rhabdomyosarcoma via the combination therapy with FOXM1 inhibitor RCM1 Frontiers in oncology 2023-02-02 [PMID: 36816948]

Review for CHAC1 Antibody (NBP2-46273) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 02/01/2019

Summary

• Application: Western Blot
• Sample Tested: Huh7 cell lysate
• Species: Human
• Lot: C-02

Bioinformatics

• Gene Symbol: CHAC1