ChemR23/CMKLR1 Antibody (BZ194) - BSA Free

Immunohistochemistry-Paraffin: ChemR23/CMKLR1 Antibody (BZ194) [NBP1-43233] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse liver using ChemR23/CMKLR1 antibody (clone BZ194) at 1:200 dilution. The antibody generated an expected membrane-cytoplasmic staining in the hepatocytes.

Flow Cytometry: ChemR23/CMKLR1 Antibody (BZ194) [NBP1-43233] - Staining of resident mouse peritoneal macrophages.

Immunohistochemistry-paraffin: Rat Monoclonal ChemR23/CMKLR1 Antibody (BZ194) [NBP1-43233] - ChemR23/CMKLR1 was detected in perfusion fixed paraffin-embedded sections of mouse intestine using ChemR23/CMKLR1 Antibody (BZ194) at a dilution of 1:25 for 1 hour at room temperature. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in intestinal villi. Image validated internally.

• Reactivity: Mu
• Applications: Flow, IHC
• Clone: BZ194
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ChemR23/CMKLR1 Antibody (BZ194) - BSA Free Summary

• Immunogen: Partial synthetic peptide made to an N-terminal portion of the mouse ChemR23/CMKLR1 protein (amino acids 8-32) [UniProt P97468]
• Localization: Multi-pass membrane protein with cytoplasmic and extra-cellular domains
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CMKLR1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
• Application Notes: This antibody was tested in Flow on resident mouse peritoneal macrophages and found that it can be used at less than or equal to 0.5 ug per test (cell number should be determined empirically but can range from 10^5 to 10^8cells/test). In IHC-P it was tested on mouse liver using routine protocol with heat induced antigen retrieval employing 10mM sodium citrate buffer pH 6.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for ChemR23/CMKLR1 Antibody (BZ194) - BSA Free

• CHEMERINR
• chemokine receptor-like 1
• chemokine-like receptor 1
• ChemR23
• cmklr1
• Dez
• Gpcr27
• G-protein coupled receptor ChemR23
• G-protein coupled receptor DEZ
• MGC126105
• MGC126106
• orphan G-protein coupled receptor, Dez

Background

The 194.7.4 (BZ194)monoclonal antibody reacts to mouse Chemoattractant-like receptor 1 (CMKLR1), also known as ChemR23 and DEZ. CMKLR1 is the receptor for chemerin, a potent chemoattractant. The BZ194 antibody was generated against the amino terminal residues of mouse CMKLR1. In the human CMKLR is found on pDC while in the mouse expression is confined to resident peritoneal macrophages with highest expression on Cd11blo macrophages. Expression is absent on in vitro cultured bone marrow DCs (except on early DCs as defined by CD11c+MHCIIint), and on freshly isolated CD11c+ cells from lymph node, spleen and bone marrow. Mouse CMKLR is thought to play a role in macrophage function as a result of injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ChemR23/CMKLR1 Antibody (NBP1-43233)(2)

Publications using NBP1-43233

• Sharma M, Schlegel MP, Afonso MS et al Regulatory T Cells License Macrophage Pro-Resolving Functions During Atherosclerosis Regression Circ Res 2020-04-28 [PMID: 32336197] (ICC/IF, ICC/IF, Mouse)
• Suzuki T, Tatsuno K, Ito T et al. Distinctive downmodulation of plasmacytoid dendritic cell functions by vitamin D3 analogue calcipotriol. J. Dermatol. Sci. 2016-06-03 [PMID: 27342039] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: CMKLR1
• Entrez:
  • 14747(Mouse)
• Uniprot:
  • P97468(Mouse)