Chikungunya Virus E1 Antibody (988122) [Unconjugated]

Western blot shows lysates of recombinant Chikungunya Virus (CHIKV) E1. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB97792) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Chikungunya Virus E1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: V
• Applications: WB
• Clone: 988122
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Chikungunya Virus E1 Antibody (988122) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived transfected with viral Chikungunya Virus E1
  Tyr810-Gly1224
  Accession # Q8JUX5
• Specificity: Detects viral Chikungunya Virus E1 in ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chikungunya Virus E1 Antibody (988122) [Unconjugated]

• CHICKV E1
• Chikungunya Virus E1
• Chikungunya Virus
• CHIKV-E1

Background

Chikungunya fever is a pandemic disease caused by the flavivirus Chikungunya (CHIKV). The 47 kDa E1 glycoprotein mediates viral membrane fusion during CHIKV infection. The CHIKV envelope protein E1 is a component of the viral spike, which is composed of triplets of heterodimer of E1 and E2 glycoproteins, expressed on the viral surface. The viral spike proteins facilitate attachment to cell surfaces and viral entry into the cells. The E1 envelope protein is a class II fusion protein that mediates low pH-triggered membrane fusion during virus infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chikungunya Virus E1 Antibody (MAB97792)(1)

Publication using MAB97792

• Judith Mary Reyes Ballista, Kerri L. Miazgowicz, Marissa D. Acciani, Ariana R. Jimenez, Ryan S. Belloli, Katherine E. Havranek et al. Chikungunya virus entry and infectivity is primarily facilitated through cell line dependent attachment factors in mammalian and mosquito cells Frontiers in Cell and Developmental Biology 2023-01-20 [PMID: 36743418]

Bioinformatics

• Uniprot:
  • Q8JUX5()