CHMP4C Antibody

Western Blot: CHMP4C Antibody [NBP2-15888] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP4C antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

Immunocytochemistry/Immunofluorescence: CHMP4C Antibody [NBP2-15888] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CHMP4C stained by CHMP4C antibody diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: CHMP4C Antibody [NBP2-15888] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

Western Blot: CHMP4C Antibody [NBP2-15888] - Sample (20 ug of whole cell lysate) A: HeLa B: HeLa cytoplasm, 12% SDS PAGE gel, diluted at 1:1000.

Western Blot: CHMP4C Antibody [NBP2-15888] - Sample (50 ug of whole cell lysate) A: Mouse Kidney, 12% SDS PAGE gel, diluted at 1:1000.

Product Discontinued

CHMP4C Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CHMP4C. The exact sequence is proprietary.
• Localization: Cytoplasm; cytosol; Late endosome membrane
• Predicted Species: Rat (91%), Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHMP4C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for CHMP4C Antibody

• charged multivesicular body protein 4c
• CHMP4c
• chromatin modifying protein 4C
• Chromatin-modifying protein 4c
• hSnf7-3
• hVps32-3
• MGC22825
• Shax3
• SNF7 homolog associated with Alix 3
• Snf7 homologue associated with Alix 3
• SNF7-3
• Vacuolar protein sorting-associated protein 32-3
• vps32-3
• VPS32C

Background

CHMP4C belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CHMP4C
• Uniprot:
  • Q96CF2(Human)