ChT1 antigen Antibody (CT1) [FITC]

Flow Cytometry: ChT1 antigen Antibody (CT1) [FITC] [NBP1-28356] - 1 ug/10^6 Chicken thymocytes were double stained with mouse anti-chicken CD3-PE and mouse anti-chicken ChT1-FITC. Analysis was performed on a flow cytometer using a light scatter gate to identify small lymphocytes.

• Reactivity: Av, Ch
• Applications: Flow, Func
• Clone: CT1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

ChT1 antigen Antibody (CT1) [FITC] Summary

• Immunogen: Chicken thymocytes
• Specificity: Chicken/Quail ChT1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • In vitro assay

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for ChT1 antigen Antibody (CT1) [FITC]

• ChT1 thymocyte antigen
• V set and immunoglobulin domain containing
• VSIG1

Background

ChT1, a member of the Ig superfamily with one V-like and one C2-like domain, is a T cell antigen that is expressed on the surface of embryonic thymocytes (day 10). In young chickens, about 90% of the thymocytes as well as a subpopulation of peripheral lymphocytes (which represent recent thymic emigrants) are ChT1 positive.1-4 Expression in the periphery declines with age and, in correlation with partial thymectomy, indicates that ChT1 can be used as an accurate marker for studying thymic function. (3) CT1 can block T cell differentiation in vitro, in thymic organ cultures and in thymocyte precursors cultured on stromal cell monolayers. (4) CT1 also recognizes quail cortical thymocytes. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.