CIITA Antibody - (1-333 aa)

Western Blot: CIITA Antibody [NBP1-77806] - Lysates of Cos-7 cells after transient transfection, separately, with pcDNA3-FLAG-CIITA-336-884 and pcDNA3-HA-CIITA.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CIITA Antibody - (1-333 aa) Summary

• Description: This antibody was prepared from monospecific antiserum by delipidation and defibrination.
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: The immunogen used for this study was a bacterially produced recombinant FLAG-CIITA corresponding to amino acids 1 through 333 of the human protein. (Uniprot: P33076)
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CIITA
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:25000
  • Western Blot 1:500-1:3000
• Application Notes: This product has been tested in western blot. For immunoblotting a 1:500 dilution is recommended. Researchers should determine optimal titers for other applications.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: 0.01% Sodium Azide
• Purity: Delipidation and Defibrination

Alternate Names for CIITA Antibody - (1-333 aa)

• C2TA
• C2TAMHC class II transactivator type III
• CIITAIV
• class II, major histocompatibility complex, transactivator
• MHC class II transactivator
• MHC2TANLR family, acid domain containing
• NLRA
• nucleotide-binding oligomerization domain, leucine rich repeat and acid domaincontaining

Background

The transactivator CIITA regulates basal and interferon-induced expression of Major Histocompatibility Complex class II genes. CIITA restores expression of all MHC class II gene expression in mutant cells and corrects regulatory defects of MHC class II genes. Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression. Antigen can be obtained from Raji cell lysates. Typically levels of CIITA expression are too low to detect endogenous levels of protein expression. Transiently transfected cells are usually employed to study this transcription factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CIITA
• Uniprot:
  • P33076(Human)