Clathrin Heavy Chain 1/CHC17 Antibody (TD.1) - BSA Free

Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (TD.1) - BSA Free [NB120-11331] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-Clathrin Heavy Chain Clone: TD.1. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. SH-SY5Y 4. SK-N-SH 5. COS7 6. PC12 7. Neuro-2a

Product Discontinued

Clathrin Heavy Chain 1/CHC17 Antibody (TD.1) - BSA Free Summary

• Immunogen: Full length native protein (purified) (Cow). UniProt accession no. Q00610.
• Epitope: The epitope recognized by the antibody resides within amino acids 207-264 of bovine CHC.
• Localization: Cell Membrane and Cytoplasmic
• Marker: Clathrin-coated Vesicle Marker
• Specificity: Monoclonal Anti-Clathrin Heavy Chain reacts specifically with the clathrin heavy chain (CHC17), and does not cross-react with the muscle isoform CHC22. The epitope recognized by the antibody resides within amino acids 207-264 of bovine clathrin heavy chains. It is reported to be non-reactive with native clathrin in immunocytochemistry and immunoprecipitation (but some staining is seen in Rat1 cells, following 4% paraformaldehyde-0.5% Triton X-100 fixation).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLTC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: WB: Use at a concentration of 1 - 4 ug/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 192 kDa). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.9% Sodium Azide
• Concentration: 2.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Clathrin Heavy Chain 1/CHC17 Antibody (TD.1) - BSA Free

• CHC
• CHC17
• Clathrin Heavy Chain 1
• Clathrin heavy chain on chromosome 17
• clathrin, heavy chain (Hc)
• clathrin, heavy chain
• clathrin, heavy polypeptide (Hc)
• clathrin, heavy polypeptide-like 2
• CLH17
• CLH-17
• CLTC
• CLTCL2
• Hc
• KIAA0034

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains (192 kDa) and three light chains (LCa or LCb, 25-29 kDa) and self assembles into a polyhedral cytoskeletal element. Self assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Clathrin Heavy Chain 1/CHC17 Antibody (NB120-11331)(1)

Publication using NB120-11331

• Grossman GH, Ebke LA, Beight CD et al. Protein partners of dynamin-1 in the retina. Vis Neurosci 2013-06-10 [PMID: 23746204]

Bioinformatics

• Gene Symbol: CLTC
• Entrez:
  • 1213(Human)
  • 67300(Mouse)
  • 54241(Rat)