CNPase Antibody (1H10) - BSA Free

Western Blot: CNPase Antibody (1H10) [NBP2-50031] - Western blot analysis of different tissue lysates using mouse mAb to CNP, NBP2-50031, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. A doublet of bands at 46 and 48kDa correspond to the major isotypes of the CNP protein.

Immunocytochemistry/Immunofluorescence: CNPase Antibody (1H10) [NBP2-50031] - Mixed neuron-glial cell cultures stained with NBP2-50031 (red) and polyclonal antibody against GFAP (green, NB300-141). NBP2-50031 stains strongly in oligodendrocytes, whereas GFAP labels only the intermediate filaments in astrocytes. Blue is DNA staining.

Immunohistochemistry Free-Floating: CNPase Antibody (1H10) [NBP2-50031] - Analysis of rat cerebellum section stained with mouse mAb to CNP, NBP2-50031, dilution 1:500 in green and costained with chicken pAb to NF-M, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The CNP antibody specifically stains oligodendrocytes, cells that create the myelin sheath around axons. NF-M antibody labels axons of neuronal cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC
• Clone: 1H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CNPase Antibody (1H10) - BSA Free Summary

• Immunogen: Full length human recombinant CNPase protein expressed in and purified from E. coli. [UniProt# P09543]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry
  • Western Blot 1:5000 - 1:20000
• Application Notes: In western blot, double bands may be detected at 46 and 48 kDa corresponding to the major isotypes of the CNP protein.
• Theoretical MW: 46,48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 0.035% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CNPase Antibody (1H10) - BSA Free

• 2', 3' cyclic nucleotide 3' phosphohydrolase
• 2'-3'-cyclic nucleotide 3' phosphodiesterase
• 2'-3'-cyclic-nucleotide 3'-phosphodiesterase
• CNP1
• CNPase
• EC 3.1.4.37

Background

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNP
• Uniprot:
  • P09543(Human)