Collagen I alpha 1 Antibody [Unconjugated]

Western blot shows lysates of human kidney tissue, human skin tissue, and human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pro-Collagen I alpha 1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of IMR‑90 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Collagen I at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Pro-Collagen I alpha 1 was detected in immersion fixed IMR-90 human lung fibroblast cell line using Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Human N-Pro Collagen I was immunoprecipitated from 500 μg of IMR‑90 human lung fibroblast cell line lysates with 5 μg of Sheep Anti-Human N-Pro Collagen I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220). The N-Pro Collagen I-antibody complexes were absorbed using Protein A or Protein G. Immunoprecipitated human N-Pro Collagen I was detected by Western blot using 1 μg/mL of Mouse Anti-Human N-Pro Collagen I Monoclonal Antibody (MAB6220) under reducing conditions and using Western Blot Buffer Group 1.

Simple Western lane view shows lysates of IMR‑90 human lung fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Collagen I alpha 1 at approximately 232 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Pro Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IP, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Collagen I alpha 1 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Collagen I
  Gln23-Lys277, Gly1094-Leu1464
  Accession # P02452
• Specificity: Detects human Pro-Collagen I alpha 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human COL3A1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: COL1A1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunoprecipitation 5 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Collagen I alpha 1 Antibody [Unconjugated]

• Alpha-1 type I collagen
• COL1A1
• COL-IA1
• Collagen 1 alpha 1
• Collagen 1
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain
• Collagen I alpha 1
• collagen of skin, tendon and bone, alpha-1 chain
• Collagen Type I Alpha 1 Chain
• collagen, type I, alpha 1
• Collagen1
• Collagen-1
• EDSARTH1
• OI4
• pro-alpha-1 collagen type 1
• Type I Procollagen Alpha 1 Chain

Background

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides (1). The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues (1). Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described (2). This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

1. Canty, E.G. et al. (2005) J. Cell Sci. 118:1341.
2. Han, S. et al. (2008) J. Mol. Biol. 383:122.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen I alpha 1 Antibody (AF6220)(24)

Publications using AF6220

• Faust, HJ;Cheng, TY;Korsunsky, I;Watts, GFM;Gal-Oz, ST;Trim, WV;Kongthong, S;Jonsson, AH;Simmons, DP;Zhang, F;Padera, R;Chubinskaya, S;Accelerating Medicines Partnership: RA/SLE Network, ;Wei, K;Raychaudhuri, S;Lynch, L;Moody, DB;Brenner, MB; Adipocyte associated glucocorticoid signaling regulates normal fibroblast function which is lost in inflammatory arthritis Nature communications 2024-11-14 [PMID: 39543086] (Immunohistochemistry, Human)
• Jocher, J;Edenhofer, FC;Müller, S;Janssen, P;Briem, E;Geuder, J;Enard, W; Generation and characterization of two Vervet monkey induced pluripotent stem cell lines derived from fibroblasts Stem cell research 2024-01-17 [PMID: 38277709] (ICC, Human)
• Jocher, J;Edenhofer, FC;Müller, S;Janssen, P;Briem, E;Geuder, J;Enard, W; Generation and characterization of two fibroblast-derived Baboon induced pluripotent stem cell lines Stem cell research 2024-01-17 [PMID: 38246118] (Immunocytochemistry, Primate - Papio anubis (Olive Baboon))
• Park, S;Park, CW;Eom, JH;Jo, MY;Hur, HJ;Choi, SK;Lee, JS;Nam, ST;Jo, KS;Oh, YW;Lee, J;Kim, S;Kim, DH;Park, CY;Kim, SJ;Lee, HY;Cho, MS;Kim, DS;Kim, DW; Preclinical and dose-ranging assessment of hESC-derived dopaminergic progenitors for a clinical trial on Parkinson's disease Cell stem cell 2023-12-07 [PMID: 38086390] (Immunohistochemistry, Xenograft)
• Maimaitili, M;Chen, M;Febbraro, F;Ucuncu, E;Kelly, R;Niclis, JC;Christiansen, JR;Mermet-Joret, N;Niculescu, D;Lauritsen, J;Iannielli, A;Klæstrup, IH;Jensen, UB;Qvist, P;Nabavi, S;Broccoli, V;Nykjær, A;Romero-Ramos, M;Denham, M; Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells Nature communications 2023-12-05 [PMID: 38052784] (Immunohistochemistry, Human, Xenograft)
• Faust, HJ;Cheng, TY;Korsunsky, I;Watts, GFM;Gal-Oz, ST;Trim, W;Kongthong, K;Jonsson, AH;Simmons, DP;Zhang, F;Padera, R;Chubinskaya, S;Accelerating Medicines Partnership Program: Rheumatoid Arthritis and Systemic Lupus Erythematosus (AMP RA/SLE) Network, ;Wei, K;Raychaudhuri, S;Lynch, L;Moody, DB;Brenner, MB; Adipocytes regulate fibroblast function, and their loss contributes to fibroblast dysfunction in inflammatory diseases bioRxiv : the preprint server for biology 2023-05-18 [PMID: 37292637] (IHC, Human)
• Jana Rájová, Marcus Davidsson, Martino Avallone, Morgan Hartnor, Patrick Aldrin-Kirk, Tiago Cardoso et al. Deconvolution of spatial sequencing provides accurate characterization of hESC-derived DA transplants in vivo Molecular Therapy - Methods & Clinical Development 6/8/2023 [PMID: 37251982]
• Ryoo, H;Underhill, GH; Combinatorial Microgels for 3D ECM Screening and Heterogeneous Microenvironmental Culture of Primary Human Hepatic Stellate Cells bioRxiv : the preprint server for biology 2023-05-08 [PMID: 37214995] (ICC, Human)
• Z You, L Wang, H He, Z Wu, X Zhang, S Xue, P Xu, Y Hong, M Xiong, W Wei, Y Chen Mapping of clonal lineages across developmental stages in human neural differentiation Cell Stem Cell, 2023-03-17;0(0):. 2023-03-17 [PMID: 36933556] (IHC, Human)
• Z You, L Wang, H He, Z Wu, X Zhang, S Xue, P Xu, Y Hong, M Xiong, W Wei, Y Chen Mapping of clonal lineages across developmental stages in human neural differentiation Cell Stem Cell, 2023;0(0):. 2023 [PMID: 36933556] (IHC, Human)
• Galina V. Beznoussenko, Hee-Seok Kweon, Irina S. Sesorova, Alexander A. Mironov Comparison of the Cisterna Maturation-Progression Model with the Kiss-and-Run Model of Intra-Golgi Transport: Role of Cisternal Pores and Cargo Domains † International Journal of Molecular Sciences 3/25/2022 [PMID: 35408951]
• JW Foster, RN Parikh, J Wang, KS Bower, M Matthaei, S Chakravart, AS Jun, CG Eberhart, US Soiberman Transcriptomic and Immunohistochemical Analysis of Progressive Keratoconus Reveal Altered WNT10A in Epithelium and Bowman's Layer Investigative Ophthalmology & Visual Science, 2021-05-03;62(6):16. 2021-05-03 [PMID: 33988693] (Western Blot, Human)
• C Tian, Y Huang, KR Clauser, S Rickelt, AN Lau, SA Carr, MG Vander Hei, RO Hynes Suppression of pancreatic ductal adenocarcinoma growth and metastasis by fibrillar collagens produced selectively by tumor cells Nature Communications, 2021-04-20;12(1):2328. 2021-04-20 [PMID: 33879793] (IHC, Western Blot, Human)
• VR Rao, EB Stubbs TGF-&beta2 Promotes Oxidative Stress in Human Trabecular Meshwork Cells by Selectively Enhancing NADPH Oxidase 4 Expression Investigative Ophthalmology & Visual Science, 2021-04-01;62(4):4. 2021-04-01 [PMID: 33821883] (ICC, Western Blot, Human)
• Liangcong Hu, Jing Liu, Hang Xue, Adriana C. Panayi, Xudong Xie, Ze Lin et al. miRNA-92a-3p regulates osteoblast differentiation in patients with concomitant limb fractures and TBI via IBSP/PI3K-AKT inhibition Molecular Therapy - Nucleic Acids 3/5/2021 [PMID: 33717654]
• Y Tao, SC Vermilyea, M Zammit, J Lu, M Olsen, JM Metzger, L Yao, Y Chen, S Phillips, JE Holden, V Bondarenko, WF Block, TE Barnhart, N Schultz-Da, K Brunner, H Simmons, BT Christian, ME Emborg, SC Zhang Autologous transplant therapy alleviates motor and depressive behaviors in parkinsonian monkeys Nature Medicine, 2021-03-01;0(0):. 2021-03-01 [PMID: 33649496] (ICC, IHC, Primate - Rhesus macaque)
• J Piao, S Zabierowsk, BN Dubose, EJ Hill, M Navare, N Claros, S Rosen, K Ramnarine, C Horn, C Fredrickso, K Wong, B Safford, S Kriks, A El Maarouf, U Rutishause, C Henchcliff, Y Wang, I Riviere, S Mann, V Bermudez, S Irion, L Studer, M Tomishima, V Tabar Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01 Cell Stem Cell, 2021-02-04;28(2):217-229.e7. 2021-02-04 [PMID: 33545080] (IHC, Mouse)
• Z Wang, ZX Cheng, ST Abrams, ZQ Lin, ED Yates, Q Yu, WP Yu, PS Chen, CH Toh, GZ Wang Extracellular histones stimulate collagen expression in vitro and promote liver fibrogenesis in a mouse model via the TLR4-MyD88 signaling pathway World Journal of Gastroenterology, 2020-12-21;26(47):7513-7527. 2020-12-21 [PMID: 33384551] (Western Blot, Human)
• Nai-Chen Cheng, Yuan-Kun Tu, Ning-Hsu Lee, Tai-Horng Young Influence of Human Platelet Lysate on Extracellular Matrix Deposition and Cellular Characteristics in Adipose-Derived Stem Cell Sheets Frontiers in Cell and Developmental Biology 10/22/2020 [PMID: 33195191]
• Rahmeh Othman, Simon Berbari, Elvire Vaucher, Réjean Couture Differential Expression of Kinin Receptors in Human Wet and Dry Age-Related Macular Degeneration Retinae Pharmaceuticals (Basel) 6/24/2020 [PMID: 32599742]
• P Vijayaraj, A Minasyan, A Durra, S Karumbayar, M Mehrabi, CJ Aros, SD Ahadome, DW Shia, K Chung, JM Sandlin, KF Darmawan, KV Bhatt, CC Manze, MK Paul, DC Wilkinson, W Yan, AT Clark, TM Rickabaugh, WD Wallace, TG Graeber, R Damoiseaux, BN Gomperts Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule Cell Rep, 2019-12-10;29(11):3488-3505.e9. 2019-12-10 [PMID: 31825831] (ICC, Western Blot, Human)
• Bose C, Megyesi J, Shah S, Hiatt K, Hall K, Karaduta O, Swaminathan S Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis. PLoS ONE, 2015-08-25;10(8):e0136563. 2015-08-25 [PMID: 26305890] (Western Blot, Human)
• Mogami , Haruta, Keller , Patrick, Shi , Haolin, Word , R Ann Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth. J Biol Chem, 2014-03-20;289(19):13295-307. 2014-03-20 [PMID: 24652285] (Western Blot, Human)
• PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation. J. Cell. Sci., 2012-02-17;125(0):2276-87. 2012-02-17 [PMID: 22344267] (Western Blot, Human)

FAQs for Collagen I alpha 1 Antibody (AF6220). (Showing 1 - 4 of 4 FAQs).

1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
   • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
   • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
3. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
   • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
4. How long does delivery take for your collagen 1 alpha 1 antibody?
   • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

Bioinformatics

• Gene Symbol: COL1A1
• Uniprot:
  • P02452()