Collagen II Antibody (2) - BSA Free

Immunohistochemistry-Paraffin: Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Staining of a formalin-fixed, paraffin-embedded biopsy from a chondroblastoma, showing specific staining of tumor cells with NBP1-05169 developed with peroxidase-labeled goat anti-mouse Ig (blue color).

Immunohistochemistry-Paraffin: Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Staining of a formalin-fixed, paraffin-embedded biopsy from a chondroblastoma, showing specific staining of tumor cells with NBP1-05169 developed with peroxidase-labeled goat anti-mouse Ig (blue color).

Immunohistochemistry: Collagen II Antibody (003-02) - BSA Free [NBP1-05169] - Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Positive stain of Canine Chondrocytes incubated overnight at room temperature at 1:100 dilution. Image courtesy of Dragana Ponjevic, Dr. Matyas Laboratory, University of Calgary.

Immunohistochemistry: Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Human chondrocytes stained with NBP1-05169 overnight at room temperature, 1:100 dilution. Image courtesy of Dragana Ponjevic, Dr. Matyas Laboratory, University of Calgary.

Immunohistochemistry-Paraffin: Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Staining of a formalin-fixed, paraffin-embedded fetal bone anlage in the hand (around week 10).

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: 2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Collagen II Antibody (2) - BSA Free Summary

• Immunogen: Recombinant expressed PIIANP
• Epitope: Epitope is located at the amino-terminal part of procollagen type IIA
• Specificity: Procollagen type IIA N-terminal propeptide
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL2A1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000
• Application Notes: ELISA: NBP1-05169 also reacts with recombinant PIIANP coated directly onto the microtiter well. IHC: NBP1-05169 diluted in 10%FCS/TBS is well suited for immunohistochemistry on formalin-fixed, paraffin-embedded fetal tissue. Use in Canine reported by customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Collagen II Antibody (2) - BSA Free

• Alpha-1 type II collagen
• ANFH
• AOM
• cartilage collagen
• Chondrocalcin
• COL11A3
• COL2A1
• Collagen 2
• collagen alpha-1(II) chain
• Collagen II
• collagen II, alpha-1 polypeptide
• collagen type 2
• collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
• collagen, type II, alpha 1
• Collagen-2
• MGC131516
• SEDC

Background

Collagen type II is the most abundant protein of cartilage matrix. Two isoforms exist and collagen type IIA is involved in early development of cartilage in embryos. The collagen is produced from type II procollagen when two teloterminal propeptides are digested from the procursor and released into the circulation. Procollagen type II N-terminal propeptide (PIIANP) is considered a marker of cartilage synthesis and the normal concentration in serum is approximately 30ng/mL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen II Antibody (NBP1-05169)(3)

Publications using NBP1-05169

• Zhang J, Sun J, Chen D et al. Suppression of matrix degradation and amelioration of disc degeneration by a 970-nm diode laser via inhibition of the p38 MAPK pathway in a rabbit model Lasers in medical science 2023-01-31 [PMID: 36717466]
• Maeda S, matsumoto m, Segawa K et al. Development of scaffold-free tissue-engineered constructs with serum-free media from mesenchymal stem cell-derived cells for cartilage repair and long-term preservation Research Square 2023-10-31 (IHC)
• Maeda S, Matsumoto M, Segawa K et Al. Development of scaffold-free tissue-engineered constructs derived from mesenchymal stem cells with serum-free media for cartilage repair and long-term preservation Cytotechnology 2024-10-01 [PMID: 39188648]

Review for Collagen II Antibody (NBP1-05169) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 08/21/2012

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Dog cartillage and bone; Human chondroma
• Species: Human
• Lot: G0212

Bioinformatics

• Gene Symbol: COL2A1