Collagen II Antibody (5B2.5) - BSA Free

Western Blot: Collagen II Antibody (5B2.5) [NB600-844] - Expression levels of related genes during differentiation induction in the 2D environment. Expression of ACAN, collagen II, collagen X and Runx2 was detected by western blotting on day 10 during induction. Image collected and cropped by Citeab from the following publication (Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system. Cell Mol Biol Lett (2019)) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. Collagen II immune-histochemical staining on cells after inoculation onto slides. Image collected and cropped by Citeab from the following publication (Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system. Cell Mol Biol Lett (2019)) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.

Expression levels of related genes during differentiation induction in the 2D environment. (a) qRT-PCR analysis of Sox9, ACAN & collagen II on days 7, 14 & 21 during induction. (b) qRT-PCR analysis of collagen X, Runx2 & annexin V on days 7, 14 & 21 during induction. The results were normalized to B2M mRNA expression. Values are means ± SD (n = 3). (c) Expression of ACAN, collagen II, collagen X & Runx2 was detected by western blotting on day 10 during induction. (d) Expression of ACAN, collagen II, collagen X & Runx2 was detected by western blotting on day 21 during induction. Significant differences from the control group (non-transfection cells) are indicated by *p < 0.05 or **p < 0.01; differences between IHH & SHH transfection groups are indicated by #p < 0.05 or ##p < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30858866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression levels of related genes during differentiation induction in the RCCS environment. (a) qPCR analysis of Sox9, ACAN & collagen II on days 7, 14 & 21 during induction. (b) qRT-PCR analysis of collagen X, Runx2, & annexin V on days 7, 14 & 21 during induction. The results were normalized to B2M mRNA expression. Values are means ± SD (n = 3). (c) Expression of ACAN, collagen II, collagen X & Runx2 was detected by western blotting on day 10 during differentiation induction. (d) Expression of ACAN, collagen II, collagen X & Runx2 was detected by western blotting on day 21 during differentiation induction. Significant differences from the control group (non-transfection cells) are indicated by *p < 0.05 or ** p < 0.01; differences between IHH & SHH transfection groups are indicated by #p < 0.05 or ##p < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30858866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. (a) Cellular morphology. (b) Toluidine blue staining on cell-microcarrier complexes. (c) Toluidine blue staining on cells after inoculation onto slides. (d) Collagen II immunohistochemical staining on cell-microcarrier complexes. (e) Collagen II immune-histochemical staining on cells after inoculation onto slides. (f) Annexin V-Cy3 immunofluorescence staining. (Bar = 100 um) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30858866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] - Staining results for the process of differentiation induction in the 2D environment. (a) Toluidine blue staining; (b) Collagen II immunohistochemical staining; (c) Annexin V-Cy3 immunofluorescence staining. (Bar = 100 um) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30858866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Ch, Rb
• Applications: WB, Simple Western, ICC/IF, IHC
• Clone: 5B2.5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Collagen II Antibody (5B2.5) - BSA Free Summary

• Immunogen: A purified preparation of lathyritic type II collagen from embryonic chicken sternum
• Localization: Cartilage matrix
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL2A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:2000
  • Simple Western 1:50
  • Western Blot 1-2 ug/ml

Reactivity Notes

Use in Rabbit reported in scientific literature (PMID:35441114).Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Rabbit reactivity reported in scientific literature (PMID:33120203).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Collagen II Antibody (5B2.5) - BSA Free

• Alpha-1 type II collagen
• ANFH
• AOM
• cartilage collagen
• Chondrocalcin
• COL11A3
• COL2A1
• Collagen 2
• collagen alpha-1(II) chain
• Collagen II
• collagen II, alpha-1 polypeptide
• collagen type 2
• collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
• collagen, type II, alpha 1
• Collagen-2
• MGC131516
• SEDC

Background

Highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In western blotting, 5B2.5 MAb reacts with the TC fragment of lathritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen II Antibody (NB600-844)(53)

Publications using NB600-844

• Zhang W, Wang H, Chu G et al. Mechanically conditioned multilayered angle-ply collagen scaffolds promote annulus fibrosus regeneration Applied Materials Today 2023-04-01 (IHC, Rat)
• Kamakura T, Jin Y, Nishio M et al. Collagen X is dispensable for hypertrophic differentiation and endochondral ossification of human iPSC derived chondrocytes JBMR Plus 2023-02-21 [PMID: 37197316] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, Mouse)
• Jiang X, Kong Y, Kuss M et al. 3D bioprinting of multilayered scaffolds with spatially differentiated ADMSCs for rotator cuff tendon-to-bone interface regeneration Applied Materials Today 2022-06-01
• He W, Wang W, Deng J et al. L-NMMA AND 1400W, INHIBITORS OF iNOS, ATTENUATE THE INDUCTION OF iNOS AND NO IN PRIMARY RABBIT COSTAL CHONDROCYTES BY FLUORIDE Fluoride 2020-01-01 (WB, Rabbit)
• Saranya M, da Silva AM, Karjalainen H et al. Magnetic-Responsive Carbon Nanotubes Composite Scaffolds for Chondrogenic Tissue Engineering Advanced healthcare materials 2023-09-02 [PMID: 37660271] (IHC, Human)
• Takács R, Vágó J, Póliska S et al. The temporal transcriptomic signature of cartilage formation Nucleic acids research 2023-05-08 [PMID: 36987858] (IHC-P, Chicken)
  
  Details:
  1:500 IHC-P dilution
• Dong Y, Sun X, Zhang Z et al. Regional and sustained dual-release of growth factors from biomimetic tri-layered scaffolds for the repair of large-scale osteochondral defects Applied Materials Today 2020-06-01 (IF/IHC)
• Janssen L, Saranya M, Leinonen M et al. Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation Carbon 2019-11-01 (IF/IHC, Human)
• He Q, Liao Y, Zhang H et Al. Gel microspheres enhance the stemness of ADSCs by regulating cell-ECM interaction Biomaterials 2024-06-01 [PMID: 38776592]
• Wang YH, Zhou Y, Gao X et Al. Duhuo Jisheng Decoction regulates intracellular zinc homeostasis by enhancing autophagy via PTEN/Akt/mTOR pathway to improve knee cartilage degeneration PLoS One 2024-01-04 [PMID: 38166086]
• Lee KW, Chung K, Nam DH et al. Decellularized allogeneic cartilage paste with human costal cartilage and crosslinked hyaluronic acid-carboxymethyl cellulose carrier augments microfracture for improved articular cartilage repair Acta biomaterialia 2023-10-07 [PMID: 37813156]
• Huang D, Li Y, Ma Z et al. Collagen hydrogel viscoelasticity regulates MSC chondrogenesis in a ROCK-dependent manner Science advances 2023-02-10 [PMID: 36763657] (IHC, Rabbit)
• Wan JT, Qiu XS, Fu ZH et al. Tumor necrosis factor-alpha inhibition restores matrix formation by human adipose-derived stem cells in the late stage of chondrogenic differentiation World Journal of Stem Cells 2022-11-26 [PMID: 36483847] (WB, Human)
• Wang Y, Fan A, Lu L et al. Exosome modification to better alleviates endoplasmic reticulum stress induced chondrocyte apoptosis and osteoarthritis Biochemical pharmacology 2022-11-09 [PMID: 36370754] (IHC-P, Human)
  
  Details:
  Dilution used in IHC-P 1:300
• Xu L, Zhang F, Cheng G et al. Attenuation of experimental osteoarthritis with human adipose-derived mesenchymal stem cell therapy: inhibition of the pyroptosis in chondrocytes Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2022-11-04 [PMID: 36331571]
• Wu H, Shang Y, Sun W et al. Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (BSN-GelMA) hydrogel Bioactive materials 2023-01-01 [PMID: 35441114] (IF/IHC, Rabbit)
• Li L, Chen Y, Fu Q Et al. Decellularized extracellular matrix loaded with IPFP-SC for repairing rabbit osteochondral defects American journal of translational research 2021-10-15 [PMID: 34786041] (ICC/IF, Rabbit)
• Wang J, Huang L, Yang X Et al. The Regulatory Effect of MicroRNA-101-3p on Disc Degeneration by the STC1/VEGF/MAPK Pathway Oxidative medicine and cellular longevity 2021-10-05 [PMID: 34650661] (WB, Human)
• Luo Z, Liu Z, Yu H et al. Multifunctional mesoporous polydopamine near-infrared photothermal controlled release of kartogenin for cartilage repair Materials & Design 2023-07-01
• Zhang N, Wang Y, Zhang J Et al. Controlled domain gels with a biomimetic gradient environment for osteochondral tissue regeneration Acta biomaterialia 2021-08-25 [PMID: 34454084] (IHC-P, Rabbit)
• Yang Y, Wang X, Zha K et al. Porcine fibrin sealant combined with autologous chondrocytes successfully promotes full-thickness cartilage regeneration in a rabbit model Journal of Tissue Engineering and Regenerative Medicine 2021-09-01 [PMID: 34044473] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, Mouse)
• Yu H, Liu Y, Yang X et al. Strontium Ranelate Promotes Chondrogenesis Through Inhibition of the Wnt/beta-catenin Pathway Stem Cell Res Ther 2021-05-21 [PMID: 34016181]
• Zhang W, Wang H, Yuan Z et al. Moderate mechanical stimulation rescues degenerative annulus fibrosus by suppressing caveolin-1 mediated pro-inflammatory signaling pathway International journal of biological sciences 2021-04-03 [PMID: 33867854] (ICC/IF, Rat)
• Jung H, Mclellan P, Welter JF, Akkus O Chondrogenesis Mesenchymal Stem Cells via Local Release of TGF beta 3 from Heparinized Collagen Biofabric Tissue engineering. Part A 2021-04-07 [PMID: 33827271]
• Yan L, Liu G, Wu X The umbilical cord mesenchymal stem cell-derived exosomal lncRNA H19 improves osteochondral activity through miR-29b-3p/FoxO3 axis Clinical and translational medicine 2021-01-01 [PMID: 33463060] (WB)
• Wang H, Xu Y, Wang P, et al. Cell-mediated injectable blend hydrogel-BCP ceramic scaffold for in situ condylar osteochondral repair Acta biomaterialia 2021-01-13 [PMID: 33453407] (IHC-P, Rabbit)
• Meng X, Grad S, Wen C et al. An impaired healing model of osteochondral defect in papain-induced arthritis J Orthop Translat 2021-01-13 [PMID: 33437629]
• Chen M, Li Y, Liu S et al. Hierarchical macro-microporous WPU-ECM scaffolds combined with Microfracture Promote in Situ Articular Cartilage Regeneration in Rabbits Bioactive Materials 2020-12-21 [PMID: 33426368] (IHC-P, Rabbit)
• Wang X, Lu Y, Wang W et al. Effect of different aged cartilage ECM on chondrogenesis of BMSCs in vitro and in vivo Regen Biomater 2020-12-28 [PMID: 33365144]
• Qiao Z, Lian M, Han Y et al. Bioinspired stratified electrowritten fiber-reinforced hydrogel constructs with layer-specific induction capacity for functional osteochondral regeneration Biomaterials 2020-10-17 [PMID: 33120203] (WB, Rabbit)
• Tome-Garcia J, Erfani P et al. Analysis of chromatin accessibility uncovers TEAD1 as a regulator of migration in human glioblastoma. Nat Commun 2018-01-10 [PMID: 30275445] (WB, Human)
• Juhasz T, Szentleleky E, Somogyi CS et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci. 2015-08-03 [PMID: 26230691] (WB, Chicken)
  
  Details:
  Collagen II antibody (clone 5B2.5) was used at 1:100 dilution for WB analysis of lysates from experiments involving chondrifying primary micromass cell cultures that were established from chicken embryos (Ross hybrid) of Hamburger-Hamilton stages 22–24. The cultures were treated or not with Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) PACAP 1–38 and/or transient MS.
• Zheng Z, Xu J, Tu Z et al. Promoter DNA Hypermethylation of Latexin Promotes Inflammatory Activation of Fibro-Nucleus Pulposus Cells in Intervertebral Disc Degeneration papers.ssrn.com 2023-05-22 (WB, Human)
• V�g� J, Katona �, Tak�cs R et al. Cyclic uniaxial mechanical load enhances chondrogenesis through entraining the molecular circadian clock Journal of Pineal Research 2022-11-01 [PMID: 36030553] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, Mouse)
• Jun Z, Yuping W, Yanran H et al. Human acellular amniotic membrane scaffolds encapsulating juvenile cartilage fragments accelerate the repair of rabbit osteochondral defects Bone & Joint Research 2022-06-09 [PMID: 35678202] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, Mouse)
• Jiang X, Wojtkiewicz M, Patwardhan C et al. The effects of maturation and aging on the rotator cuff tendon-to-bone interface The FASEB Journal 2022-03-18 [PMID: 34822203] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, Mouse)
• You Q, Liu Z, Zhang J et al. Human Amniotic Mesenchymal Stem Cell Sheets Encapsulating Cartilage Particles Facilitate Repair of Rabbit Osteochondral Defects Am J Sports Med 2020-01-15 [PMID: 31940211]
• Jing H, Zhang X, Luo K, et al. miR-381-abundant small extracellular vesicles derived from kartogenin-preconditioned mesenchymal stem cells promote chondrogenesis of MSCs by targeting TAOK1 Biomaterials 2020-02-01 [PMID: 31864017] (WB, Human)
• Yan L, Wu X Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity Cell Biol. Toxicol. 2019-12-09 [PMID: 31820164] (WB, Human)
• Cheng B, Liu Y, Zhao Y et al. The role of anthrax toxin protein receptor 1 as a new mechanosensor molecule and its mechanotransduction in BMSCs under hydrostatic pressure Sci Rep 2019-09-02 [PMID: 31477767] (WB, Rat)
• Zhang A, Sun L, Chen K et al. 3D printing viscoelastic hydrogel-based scaffolds with a swelling-dependent gate for cartilage injury regeneration Chemical Engineering Journal 2023-11-01 (IHC)
  
  Details:
  Scaffold
• Suo H, Li L, Zhang C et al. Glucosamine-grafted methacrylated gelatin hydrogels as potential biomaterials for cartilage repair J. Biomed. Mater. Res. Part B Appl. Biomater. 2019-08-01 [PMID: 31369700]
• Gao B, Jing H, Gao M et al. Long-segmental Tracheal Reconstruction in Rabbits with Pedicled Tissue-engineered Trachea based on a 3D-printed Scaffold Acta Biomater 2019-07-25 [PMID: 31352107] (IHC-P, Rabbit)
• Chen L, Liu G, Li W, Wu X. Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system Cell Mol Biol Lett 2019-12-01 [PMID: 30858866] (WB, Rabbit)
• Zhu L, Weng Z, Shen P et al. S100B regulates inflammatory response during osteoarthritis via fibroblast growth factor receptor 1 signaling. Mol Med Rep. 2018-10-01 [PMID: 30280200] (IF/IHC, Human)
• Chen Y, Sui J, Wang Q et al. Injectable self-crosslinking HA-SH/Col I blend hydrogels for in vitro construction of engineered cartilage Carbohydr Polym 2018-06-15 [PMID: 29628260] (IF/IHC, Rabbit)
• Lekawanvijit S, Kumfu S, Wang BH et al. The Uremic Toxin Adsorbent AST-120 Abrogates Cardiorenal Injury Following Myocardial Infarction. PLoS One 2013 Dec 13 [PMID: 24349545] (WB, Rat)
• Triulzi T, Ratti M, Tortoreto M et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer. 2013-11-14 [PMID: 24242003] (IHC-P, Human)
• Brini AT, Niada S, Lambertini E et al. Chondrogenic potential of Human mesenchymal stem cells and expression of Slug transcription factor. J Tissue Eng Regen Med 2013-07-21 [PMID: 23873763]
• Dahia CL, Mahoney E, Wylie C. Shh signaling from the nucleus pulposus is required for the postnatal growth and differentiation of the mouse intervertebral disc. PLoS One. 2012-01-01 [PMID: 22558278] (IHC-Fr, ICC/IF, Mouse)
• Wang Y, Zhou Y, Gao X et al. Duhuo Jisheng Decoction Regulates Intracellular Zinc Homeostasis by Enhancing Autophagy via PTEN/Akt/mTOR to Improve Knee Cartilage Degeneration bioRxiv 2023-08-22 (IHC, Rat)
• Chen Honglin, Malheiro Afonso de Botelho Ferreira Braga, Van Blitterswijk Clemens et al. Direct Writing Electrospinning of Scaffolds with Multidimensional Fiber Architecture for Hierarchical Tissue Engineering. ACS Omega. 2017-11-08 [PMID: 29043781] (IHC-P, Human)

Bioinformatics

• Gene Symbol: COL2A1
• Entrez:
  • 395069(Chicken)
  • 1280(Human)
  • 12824(Mouse)
• Uniprot:
  • Q90W37(Chicken)