Human Collagen II ELISA Kit (Colorimetric)

ELISA: Human Collagen II ELISA Kit (Colorimetric) [NBP2-75853] - Samples were spiked with high concentrations of Human Collagen II and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Collagen II ELISA Kit (Colorimetric) [NBP2-75853] - Samples were spiked with high concentrations of Human Collagen II and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Collagen II ELISA Kit (Colorimetric) [NBP2-75853] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL

Human Collagen II ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human COL2A1 in samples. No significant cross-reactivity or interference between Human COL2A1 and analogues was observed.
• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 4.76
• Intra-Assay: %CV < 5.04
• Spike Recovery: 85 - 101%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: COL2A1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Collagen II ELISA Kit (Colorimetric)

• Alpha-1 type II collagen
• ANFH
• AOM
• cartilage collagen
• Chondrocalcin
• COL11A3
• COL2A1
• Collagen 2
• collagen alpha-1(II) chain
• Collagen II
• collagen II, alpha-1 polypeptide
• collagen type 2
• collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
• collagen, type II, alpha 1
• Collagen-2
• MGC131516
• SEDC

Background

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: COL2A1