| Immunogen | Clinical Tissue Human Colon Normal Tissue Lysate |
| Specificity | Sex: Female Age :43 Diagnosis: Adenocarcinoma, well differentiated Grade:1 Stage:IIIB TNM:T3N1M0 |
| Preparation Method |
Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature. |
| Application Notes | These lysates are proteomic discovery tools.Researchers should validate and optimize for individual use. Potential applications MAY include WB, immunoprecipitation, protein-protein interactions, ligand binding, ELISA. Note: For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Concentration | 1 mg/ml |
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