COMP/Thrombospondin-5 Antibody (360215) [Unconjugated]

Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human COMP/Thrombospondin-5 Monoclonal Antibody (Catalog # MAB3134) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for COMP/Thrombospondin-5 at approximately 90 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 360215
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

COMP/Thrombospondin-5 Antibody (360215) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human COMP/Thrombospondin-5
  Gln21-Ala757 (Ala256Arg)
  Accession # P49747.2
• Specificity: Detects human COMP/Thrombospondin-5 in direct ELISAs and Western blots. Does not cross‑react with recombinant human Thrombospondin-1, -2, or -4.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: COMP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.05 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for COMP/Thrombospondin-5 Antibody (360215) [Unconjugated]

• cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple)
• cartilage oligomeric matrix protein
• cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple)
• COMP
• EDM1
• EPD1
• MED
• MEDMGC131819
• MGC149768
• PSACH
• pseudoachondroplasia (epiphyseal dysplasia 1, multiple)
• THBS5
• Thrombospondin5
• Thrombospondin-5
• TSP5

Background

Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). The human COMP cDNA encodes a 757 amino acid (aa) precursor that includes a 20 aa signal sequence followed by a non-collagenous coiled-coil domain, four EGF-like repeats, seven TSP type-3 repeats, and a globular TSP C-terminal domain (5). Human COMP shares 86-93% aa sequence identity with rat, mouse, equine, bovine, and canine COMP. Within the TSP type-3 repeats and TSP C-terminal domain, human COMP shares 60%, 61%, 74%, and 80% aa sequence identity with human Thrombospondin-1, -2, -3, and -4, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D₃ which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin alpha 5 beta 1, although when reduced and in the absence of calcium, attachment is mediated via Integrin alpha V beta 3 (9). COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type-3 repeat, results in diminished calcium binding ability (13, 14).

1. Adams, J.C. and J. Lawler (2004) Int J. Biochem. Cell Biol. 36:961.
2. Posey, K.L. et al. (2004) Int. J. Biochem. Cell Biol. 36:1005.
3. Adams, J.C. (2004) Int. J. Biochem. Cell Biol. 36:1102.
4. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
5. Newton, G. et al. (1994) Genomics, 24:435.
6. DiCesare, P. et al. (1995) J. Orthopaedic Res. 13:422.
7. Efimov, V.P. et al. (1994) FEBS Lett. 341:54.
8. Ozbek, S. et al. (2002) EMBO J. 21:5960.
9. Chen, F.H. et al. (2005) J. Biol. Chem. 280:32655.
10. Wislowska, M. and B. Jablonska (2005) Clin. Rheumatol. 24:278.
11. Xiao, Y. et al. (2004) J. Gastroenterol. Hepatol. 19:296.
12. Liao, Q. et al. (2003) Scand. J. Gastroenterol. 38:207.
13. Kennedy, J. et al. (2005) Eur. J. Hum. Genet. 13:547.
14. Hou, J. et al. (2000) Cell Calcium 27:309.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for COMP/Thrombospondin-5 Antibody (MAB3134). (Showing 1 - 1 of 1 FAQ).

1. I have synovial fluid sample and I want to work on Western bloting can you support me ,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?
   • We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374

Bioinformatics

• Gene Symbol: COMP
• Uniprot:
  • P49747.2()