CrkRS Antibody

Immunohistochemistry-Paraffin: CrkRS Antibody [NLS7350] - Anti-CDK12 / CRKRS antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: CrkRS Antibody [NLS7350] - Analysis of anti-CDK12 / CRKRS antibody with human breast carcinoma at 6 ug/ml.

Immunohistochemistry-Paraffin: CrkRS Antibody [NLS7350] - Anti-CDK12 / CRKRS antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Product Discontinued

CrkRS Antibody Summary

• Immunogen: Synthetic 20 amino acid peptide from internal region of human CDK12 / CRKRS.
• Localization: Nuclear
• Specificity: Human CDK12 / CRKRS. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except AKAP12 (55%).
• Predicted Species: Rat (90%), Bat (90%), Rabbit (95%), Bovine (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDK12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 6 ug/ml

Reactivity Notes

Gorilla, Gibbon, Marmoset. Predicted cross-reactivity based on sequence identity: Elephant (95%), Mouse (85%), Hamster (85%), Equine (85%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CrkRS Antibody

• Cdc2-related kinase, arginine/serine-richCrkRS
• Cell division cycle 2-related protein kinase 7
• Cell division protein kinase 12
• CRK7CDC2-related protein kinase 7
• CRKR
• CRKRS
• cyclin-dependent kinase 12
• EC 2.7.11
• EC 2.7.11.23
• hCDK12
• KIAA0904EC 2.7.11.22

Background

CRK7 is a CDC2/CDK -type protein kinase. Also known as CRKRS, the protein has an arginine/serine-rich (RS) domain that is found predominantly in splicing factors, and extensive proline-rich regions that match the consensus for SH3 and WW domain binding sites. Anti-CrkRS immunoprecipitates have been shown to phosphorylate the C-terminal domain of RNA polymerase II in vitro. CRK7 has been shown to be expressed ubiquitously. ESTs have been isolated from several tissue libraries, including normal lymph and cancerous bone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CrkRS Antibody (NLS7350)(1)

Publication using NLS7350

• Capra, M, Nuciforo, P G, Confalonieri, S, Quarto, M, Bianchi, M, Nebuloni, M, Boldorini, R, Pallotti, F, Viale, G, Gishizky, M L, Draetta, G F, Di Fiore, P P. Frequent Alterations in the Expression of Serine/Threonine Kinases in Human Cancers, . 66, 8147-8154. 2006-01-01 [PMID: 16912193]

Bioinformatics

• Gene Symbol: CDK12
• Entrez:
  • 51755(Human)
• Uniprot:
  • Q9NYV4(Human)