CRMP2 Antibody

Western Blot: CRMP2 Antibody [NBP3-02989] - Western blot analysis of extracts of various cell lines, using CRMP2/CRMP2 antibody (NBP3-02989) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP3-02989] - Immunohistochemistry of paraffin-embedded rat brain using CRMP2/CRMP2 Rabbit pAb (NBP3-02989) at dilution of 1:400 (40x lens).

Knockout Validated: CRMP2 Antibody [NBP3-02989] - Western blot analysis of extracts from normal (control) and CRMP2/CRMP2 knockout (KO) HeLa cells, using CRMP2/CRMP2 antibody (NBP3-02989) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Product Discontinued

CRMP2 Antibody Summary

• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human CRMP2 (NP_001377.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DPYSL2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Knockout Validated
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 50% glycerol, pH7.3.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CRMP2 Antibody

• Collapsin response mediator protein 2
• CRMP-2
• CRMP2N2A3
• DHPRP2
• dihydropyrimidinase-like 2 long form
• dihydropyrimidinase-like 2
• dihydropyrimidinase-related protein 2
• DRP2collapsin response mediator protein hCRMP-2
• DRP-2ULIP-2
• ULIP2
• Unc-33-like phosphoprotein 2

Background

Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins involved in neuronal differentiation and axonal guidance. CRMP2 was previously shown to mediate the repulsive effect of Sema3A on axons and to participate in axonal specification. The CRMPs appear to play a complex role in axon growth as well as microtubule dynamics and axon induction. CRMPs localize to the lamellipodia and filopodia of axonal growth cones, suggesting a role in axon guidance. Moreover, CRMP2 is upregulated after axotomy, and appears to increase the formation of axon-type processes from hippocampal neurons. CRMP2 has been reported to bind tubulin dimers directly and modulate microtubule assembly. CRMPs have also been implicated in the pathogenesis of a paraneoplastic neurologic syndrome. Interaction studies have implicated phospholipase D2 (PLD2), the cytosolic tyrosine kinase Fes, and intersectin in CRMP function. Hyperphosphorylation of CRMP2 is an early event in the progression of Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPYSL2