CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free

Western Blot: CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free [NBP2-80682] - Analysis of recombinant CRTH2 (GPR44) probed with CRTH2 antibody at 1 ug/ml. Image from the standard format of this antibody.

Immunohistochemistry: CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free [NBP2-80682] - Human testis stained with CRTH2 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 54N3F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free Summary

• Immunogen: A recombinant protein containing multiple external domains of GPR44 (CRTH2) was used as immunogen for the antibody. NP_004769
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTGDR2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-5 ug/ml
• Application Notes: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free

• CD294 antigen
• CD294
• Chemoattractant receptor-homologous molecule expressed on Th2 cells
• CRTH2
• CRTH-2
• CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells
• DL1R
• DP2
• G protein-coupled receptor 44
• GPR44
• PGD2R
• PTGDR2
• putative G-protein coupled receptor 44

Background

GPR44, or G-protein coupled receptor 44, is also designated as CRTH2 and CD294. CRTH2 is expressed specifically on CD4+ Th2 cells and type 2 cytotoxic effector cells (1). CRTH2 is a prostaglandin D2 receptor PGD2 and has some similar functions to other chemokines exerting chemotaxis of Th2 cells as well as eosinophils and basophils (2). Interestingly inhibition of hair growth by PGD2 is through CRTH2 so the thought is that inhibition of CRTH2 may also eliminate hair-loss or balding effects of PGD2 . This implies that inhibitors to CRTH2 could be the new Rogaine(?) (3, 4). CRTH2 is thought to be important in allergic diseases, including asthma, and several companies are already testing drugs that block CRTH2 in clinical trials to treat these diseases (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTGDR2