CTRP7 Antibody (Anna-1)

Western Blot: CTRP7 Antibody (Anna-1) [NBP1-97528] - Analysis of mouse CTRP7 using MAb to CTRP7 (Anna-1). Method: Cell extracts (5x105) from HEK 293T( lane1) or HEK293wt transfected with a plasmid coding for mouse CTRP7 (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to CTRP7 (Anna-1) (1:1000) for 2 hours, followed by incubation with HRP-coupled anti-mouse secondary antibody (1:4000).

Western Blot: CTRP7 Antibody (Anna-1) [NBP1-97528] - Analysis of mouse CTRP7 using MAb to CTRP7 (Anna-1). Method: Cell extracts (5x105) from HEK 293T( lane1) or HEK293wt transfected with a plasmid coding for mouse CTRP7 (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to CTRP7 (Anna-1) (1:1000) for 2 hours, followed by incubation with HRP-coupled anti-mouse secondary antibody (1:4000).

Immunohistochemistry-Paraffin: CTRP7 Antibody (Anna-1) [NBP1-97528] - Staining of endogenous human CTRP7 in different human tissues (paraffin sections) using MAb to CTRP7 (Anna-1). Method: Different human normal (A: Cerabral cortex; B: Colon; C: Kidney) or cancer (D: Prostate ; E: Colo-rectal; F: Pancreatic) tissues were stained with MAb to CTRP7 (Anna-1) by standard immunohistochemistry.

Product Discontinued

CTRP7 Antibody (Anna-1) Summary

• Immunogen: Recombinant mouse CTRP7 (C1q tumor necrosis factor-alpha-related protein 7) (aa 18-290).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: C1QTNF7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:200
  • Western Blot 1:1000

Reactivity Notes

Human and Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CTRP7 Antibody (Anna-1)

• C1q and tumor necrosis factor related protein 7
• C1qTNF7
• complement-c1q tumor necrosis factor-related protein 7
• CTRP7
• CTRP7complement C1q tumor necrosis factor-related protein 7
• ZACRP7

Background

Adiponectin is involved in controlling the whole-body metabolism, particularly by increasing insulin sensitivity in muscle and liver, and by increasing fatty acid oxidation in muscle. CTRP7 belongs to a highly conserved family of adiponectin paralogs designated as C1q tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. A member of this family, CTRP2 (mouse) has been reported to rapidly induce phosphorylation of AMP-activated protein kinase, acetyl-CoA carboxylase, and mitogen-activated protein kinase in C2C12 myotubes, which resulted in increased glycogen accumulation and fatty acid oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C1QTNF7