Curcumin

Curcumin [NBP2-26243] - inhibition of PMA and TNF-a activated NF-kB signaling. NF-kB/SEAPorterTM HEK 293 (NBP2-26260) cells were plated in 12-well plates (0.5 x 10^6 cells/well) for 16 h. Cells were preincubated with different concentrations of DMSO-solubilized curcumin for 2 h or a DMSO vehicle (V) control. Cell were then stimulated with 10 ng/ml phorbol-12-myristate-13-acetate (PMA) [A] or 10 ng/ml TNF-a [B] for 24 h. The SEAPorter Assay Kit was used to measure SEAP, the readout assay for measuring NF-kB activation in TLR5/NF-kB cells. The results showed that the cells had basal level of NF-kB activity which was increased by PMA or TNF-a . They also show that curcumin decreased PMA and TNF-a activated NF-kB signaling in a dose-dependent manner

Ligand Activation: Curcumin [NBP2-26243] - Curcumin inhibition of ligand activated TLR/NF-kB signaling. TLR5/NF-kB/SEAPorterTM HEK 293 (NBP2-26277) cells were plated in 12-well plates (0.5 x 106 cells/well) for 16 h. Cells were preincubated with increasing concentrations of DMSO-solubilized curcumin (IMG-2010) for 2 h or a DMS0 vehicle (V) control. Cell were stimulated with the TLR5 ligand Flagellin (10 ng/ml: NBP2-25289 for 24 h. The SEAPorter Assay Kit was used to measure SEAP, the readout assay for measuring NF-kB activation in TLR5/NF-kB cells. The results showed that the cells had a minimal basal level of NF-kB activity which was dramatically increased by Flagellin. They also, shown that curcumin decreased Flagellin-activated NF-kB signaling in a dose-dependent manner.

Curcumin [NBP2-26243]

• Reactivity: Hu

Curcumin Summary

• Immunogen: CAS Number 458-37-7
  Linear Formula [HOC6H3(OCH3)CH=CHCO]2CH2
  Molecular Weight 368.38
  Beilstein Registry Number 2306965
  Colour Index Number 75300
  EC Number 207-280-5
  MDL number MFCD00008365
  PubChem Substance ID 24892408

Applications/Dilutions

• Application Notes: 1.Inhibition of NF-kB signaling. This includes inhibition of NF-kB activity induced by Toll-like receptor (TLR) ligands, TNF-phorbol-12-myristate-13-acetate (PMA), and hydrogen peroxide. 2. Inhibition of other cell signaling molecules including c-Jun/AP-1, Protein kinase C, MAPK, Bcl-2, COX-2, EGFR, and mTOR pathways. Additionally, curcumin can directly inhibit homodimerization of TLR4. 3. Curcumin activates certain signaling molecules including sucBax and Bcl-XS. 4. Researchers are encouraged to consult the literature regarding additional information on curcumin applications.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Reconstitution Instructions: Reconstitute with DMSO to bring curcumin to a final concentration of 11 mg/ml.

Alternate Names for Curcumin

• (E,E)-1,7-bis(4-Hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione
• Curcuma longa (Turmeric)
• Curcuma longa
• Diferuloylmethane
• Diferulylmethane
• Natural Yellow 3

Background

Curcumin, the active ingredient of the spice turmeric, is a highly pleiotropic, complex molecule which interacts with a number of signaling targets involved in inflammation and other biological processes (reviewed in Epstein et al, 2009 and Jurenka, 2009). Many signaling molecules ultimately signal through NF-kB, and whereas many pharmacological activities have been ascribed to curcumin, the inhibition of NF-kB signaling has been a key focus. In this regard, data from IMGENEX's laboratories, as well as from other model systems in the scientific literature, provide supporting evidence for curcumin as an extremely powerful research tool for inhibiting NF-kB activation. NF-kB is a ubiquitous eukaryotic transcription factor that plays a key role in regulating a number of cellular processes including inflammation, cellular proliferation, transformation and tumorigenesis. In unstimulated cells, NF-kB dimers are held in the cytoplasm by IkBs which mask the nuclear localization signals (NLS) of NF-kB required for nuclear translocation and activation. Following cell stimulation, IkB is phosphorylated and degraded. This results in NLS exposure, thereby enabling NF-kB to translocate to the nucleus and activate transcription. Evidence suggests that curcumin suppresses NF-kB activation including NF-kB driven proinflammatory gene expression by blocking I B phosphorylation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.