Recombinant Human CXCL12/SDF-1 gamma Protein

SDS-Page: Human CXCL12 gamma/SDF1 gamma Protein [NBP2-35262]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human CXCL12/SDF-1 gamma Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 99 amino acid residues corresponding to CXCL12/SDF-1 gamma Source: E. coli

  Uniprot ID: P48061

  Amino Acid Sequence: GKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKG RREEKVGKKE KIGKKKRQKK RKAAQKRKN

• Details of Functionality: CXCL12/SDF-1 gamma protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL2 activated human peripheral blood T lymphocytes is in a concentration range of 30-100 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CXCL12
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of CXCL12/SDF-1 gamma as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 11.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human CXCL12/SDF-1 gamma Protein

• CXCL12/SDF-1 gamma
• SDF1 gamma
• SDF1g

Background

CXCL12 also known as SDF1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the Cterminal tail. And all SDF1 isoforms undergo proteolytic processing of the first two Nterminal amino acids. Human SDF1gamma is synthesized as a 119 amino acid (a.a.) precursor that contains a 21 a.a. signal sequence and a 98 a.a. mature region and the SDF1gamma Cterminus binds heparin in secreted SDF1gamma, or targets the isoform to the nucleolus in the absence of a signal sequence. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for Tlymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CXCL12
• Uniprot:
  • P48061()
  • P48061()
  • P48061()