CXCL16 Antibody (142417) [Unconjugated]

Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL16 Chemokine Domain (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL16 Monoclonal Antibody (Catalog # MAB503). The ND50 is typically 0.15‑0.6 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 142417
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CXCL16 Antibody (142417) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CXCL16
  Asn27-Pro114
  Accession # Q8BSU2
• Specificity: Detects mouse CXCL16 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, recombinant mouse CXCL1, 2, 6, 9, 10, 11, 12, 13, 14, 15, recombinant rat CXCL1, 2, 3, 5, or recombinant porcine CXCL8 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CXCL16
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.15-0.6 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse CXCL16 Biotinylated Antibody (Catalog number BAF503)
  Standard: Recombinant Mouse CXCL16 Chemokine Domain (Catalog number 503-CX)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL16 Antibody (142417) [Unconjugated]

• chemokine (C-X-C motif) ligand 16
• CXC chemokine ligand 16
• CXCL16
• CXCLG16
• Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein
• SCYB16
• Small-inducible cytokine B16
• SRPSOXC-X-C motif chemokine 16
• SR-PSOXTransmembrane chemokine CXCL16

Background

Mouse CXCL16 (CXC chemokine 16) is a non-ELR motif-containing CXC chemokine with a transmembrane domain. CX3CL1/Fractalkine andCXCL16 are the only two transmembrane chemokines within the superfamily. Mouse CXCL16 cDNA encodes a 246 amino acid (aa) precursor protein with a putative 26 aa signal peptide, an 88 aa chemokine domain, an 87 aa mucin-like spacer region, a 22 aa transmembrane domain, and a 23 aa cytoplasmic tail. Mouse and human CXCL16 share 49% aa sequence identity. Mouse CXCL16 is produced by dendritic cells in lymphoid organ T cell zones and bycells in the splenic red pulp both as membrane-bound and soluble forms. Based on northern blot analysis, CXCL16 is also expressed in some non-lymphoid tissues such as lung, small intestine and kidney. The receptor for CXCL16 has been identified as CXCR6 (previously called Bonzo, STRL33 or TYMSTR), which is also a coreceptor for HIV entry. CXCR6 is expressed on naive CD8 cells, natural killer T cells and activated CD8 and CD4 T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL16 Antibody (MAB503)(16)

Publications using MAB503

• Koya, J;Tanigawa, T;Mizuno, K;Kim, H;Ito, Y;Yuasa, M;Yamaguchi, K;Kogure, Y;Saito, Y;Shingaki, S;Tabata, M;Murakami, K;Chiba, K;Okada, A;Shiraishi, Y;Marouf, A;Liévin, R;Chaubard, S;Jaccard, A;Hermine, O;de Leval, L;Tournilhac, O;Damaj, G;Gaulard, P;Couronné, L;Yasui, T;Nakashima, K;Miyoshi, H;Ohshima, K;Kataoka, K; Modeling NK-cell lymphoma in mice reveals its cell-of-origin and microenvironmental changes and identifies therapeutic targets Nature communications 2024-10-22 [PMID: 39438472] (Neutralization, Transgenic Mouse)
• Rakebrandt, N;Yassini, N;Kolz, A;Schorer, M;Lambert, K;Goljat, E;Estrada Brull, A;Rauld, C;Balazs, Z;Krauthammer, M;Carballido, JM;Peters, A;Joller, N; Innate acting memory Th1 cells modulate heterologous diseases Proceedings of the National Academy of Sciences of the United States of America 2024-06-11 [PMID: 38838013] (Neutralization, Mouse)
• Broquet, A;Gourain, V;Goronflot, T;Le Mabecque, V;Sinha, D;Ashayeripanah, M;Jacqueline, C;Martin, P;Davieau, M;Boutin, L;Poulain, C;Martin, FP;Fourgeux, C;Petrier, M;Cannevet, M;Leclercq, T;Guillonneau, M;Chaumette, T;Laurent, T;Harly, C;Scotet, E;Legentil, L;Ferrières, V;Corgnac, S;Mami-Chouaib, F;Mosnier, JF;Mauduit, N;McWilliam, HEG;Villadangos, JA;Gourraud, PA;Asehnoune, K;Poschmann, J;Roquilly, A; Sepsis-trained macrophages promote antitumoral tissue-resident T cells Nature immunology 2024-05-01 [PMID: 38684922] (In vivo assay, Mouse)
• Díez-Sánchez, A;Lindholm, HT;Vornewald, PM;Ostrop, J;Yao, R;Single, AB;Marstad, A;Parmar, N;Shaw, TN;Martín-Alonso, M;Oudhoff, MJ; LSD1 drives intestinal epithelial maturation and controls small intestinal immune cell composition independent of microbiota in a murine model Nature communications 2024-04-22 [PMID: 38649356] (Neutralization, Transgenic Mouse)
• Gnirck, AC;Philipp, MS;Waterhölter, A;Wunderlich, M;Shaikh, N;Adamiak, V;Henneken, L;Kautz, T;Xiong, T;Klaus, D;Tomczyk, P;Al-Bahra, MM;Menche, D;Walkenhorst, M;Lantz, O;Willing, A;Friese, MA;Huber, TB;Krebs, CF;Panzer, U;Kurts, C;Turner, JE; Mucosal-associated invariant T cells contribute to suppression of inflammatory myeloid cells in immune-mediated kidney disease Nature communications 2023-11-15 [PMID: 37968302] (In Vivo, Mouse)
• B Palakurthi, SR Fross, IH Guldner, E Aleksandro, X Liu, AK Martino, Q Wang, RA Neff, SM Golomb, C Lewis, Y Peng, EN Howe, S Zhang Targeting CXCL16 and STAT1 augments immune checkpoint blockade therapy in triple-negative breast cancer Nature Communications, 2023-04-13;14(1):2109. 2023-04-13 [PMID: 37055410] (IHC, Mouse)
• Anastasia du Halgouet, Aurélie Darbois, Mansour Alkobtawi, Martin Mestdagh, Aurélia Alphonse, Virginie Premel et al. Role of MR1-driven signals and amphiregulin on the recruitment and repair function of MAIT cells during skin wound healing Immunity 1/10/2023 [PMID: 36630919]
• E Klapproth, A Witt, P Klose, J Wiedemann, N Vavilthota, SR Künzel, S Kämmerer, M Günscht, D Sprott, M Lesche, F Rost, A Dahl, E Rauch, L Kattner, S Weber, P Mirtschink, I Kopaliani, K Guan, K Lorenz, P Saftig, M Wagner, A El-Armouch Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarction Nature Communications, 2022-12-10;13(1):7648. 2022-12-10 [PMID: 36496449] (Western Blot, Mouse)
• Sarah F. Rosen, Allison L. Soung, Wei Yang, Shenjian Ai, Marlene Kanmogne, Veronica A. Davé et al. Single-cell RNA transcriptome analysis of CNS immune cells reveals CXCL16/CXCR6 as maintenance factors for tissue-resident T cells that drive synapse elimination Genome Medicine 9/24/2022 [PMID: 36153630]
• A Jarade, Z Garcia, S Marie, A Demera, I Prinz, P Bousso, JP Di Santo, N Serafini Inflammation triggers ILC3 patrolling of the intestinal barrier Nature Immunology, 2022-08-23;0(0):. 2022-08-23 [PMID: 35999393] (In Vivo, Mouse)
• Chen Y, Yang S, Tavormina J et al. Oncogenic collagen I homotrimers from cancer cells bind to alpha 3 beta 1 integrin and impact tumor microbiome and immunity to promote pancreatic cancer Cancer cell 7/13/2022 [PMID: 35868307]
• S Catalano, S Panza, G Augimeri, C Giordano, R Malivindi, L Gelsomino, S Marsico, F Giordano, B Gy?rffy, D Bonofiglio, S Andò, I Barone Phosphodiesterase 5 (PDE5) Is Highly Expressed in Cancer-Associated Fibroblasts and Enhances Breast Tumor Progression Cancers (Basel), 2019-11-06;11(11):. 2019-11-06 [PMID: 31698786] (Neutralization, Mouse)
• Lepore F, D'Alessandro G, Antonangeli F et al. CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma Front Immunol 11/27/2018 [PMID: 30542347]
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• Wu T, Xie C, Bhaskarabhatla M, Yan M, Leone A, Chen SS, Zhou XJ, Putterman C, Mohan C Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance. Arthritis Rheum., 2007-03-01;56(3):949-59. 2007-03-01 [PMID: 17328072] (Neutralization, Mouse)
• Gough PJ, Garton KJ, Wille PT, Rychlewski M, Dempsey PJ, Raines EW A disintegrin and metalloproteinase 10-mediated cleavage and shedding regulates the cell surface expression of CXC chemokine ligand 16. J. Immunol., 2004-03-15;172(6):3678-85. 2004-03-15 [PMID: 15004171] (ELISA Development, Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CXCL16
• Uniprot:
  • Q8BSU2()