CXCR4 Antibody (12G5) [Unconjugated]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB170) or (B) Mouse IgG2AIsotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB170) or (B) Mouse IgG2AIsotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Recombinant Human/ Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF‑1 alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCR4 Monoclonal Antibody (Catalog # MAB170). The ND50 is typically 0.3-1.2 µg/mL.

Jurkat (filled histogram) vs K562 (open histogram) cells were stained with Mouse Anti-Human CXCR4 Monoclonal Antibody followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.

Effect of hypoxia on CX3CR1 and CXCR4 expression by early culture of MSCs.(A) The MSCs were cultured for 4–20 h in normoxic (Nor) or hypoxic (Hyp) conditions, as indicated. Total RNA was analyzed by RT-PCR for CX3CR1, CX3CR4, and b-actin mRNA expression. (B) Total RNA from MSCs cultured for 20 h in the presence of increasing concentrations of DFX was analyzed by RT-PCR for CX3CR1 and CXCR4 mRNA expression. (C) Quantitaive RT-PCR for CXCR4 and CX3CR1 mRNA expression at 20 h of treatment. The vertical axis represents the relative ratio of indicated condition to normoxic condition without the addition of DFX.. (D) Surface expression of CXCR4 and CX3CR1 were determined by flow cytometry using a mouse mAb anti-CXCR4 and a rat mAb anti-CX3CR1, respectively (continuous line). Irrelevant antibody is indicated by the dotted line. The results are representative of three independent experiments. (E) Hypoxia and DFX-treated MSCs were cultured for 24 h in the indicated conditions and analyzed by western blot for HIF-1 alpha , CXCR4, CX3CR1, and actin protein levels. (F) HIF-1 alpha binding to the CX3CR1 promoter. The MSCs were cultured for 4 h in normoxic or hypoxic condition. ChIP was performed with or without rabbit antibody specific for HIF-1 alpha . There is no difference between PCR of the CX3CR1 promoter using input chromatin for normoxic and hypoxic cells. However, a band was noted for hypoxic cells but not for normoxic cells, when PCR of the CX3CR1 promoter was performed using immunoprecipitation with HIF-1 alpha antibody. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/17476338), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 12G5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCR4 Antibody (12G5) [Unconjugated] Summary

• Immunogen: CP-MAC-infected SUP-T1 human T cell lymphoblastic lymphoma line
  Accession # P61073
• Specificity: Detects human CXCR4.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR4
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.3-1.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR4 Antibody (12G5) [Unconjugated]

• CD184
• chemokine (C-X-C motif) receptor 4
• chemokine (C-X-C motif), receptor 4 (fusin)
• C-X-C chemokine receptor type 4
• CXCR4
• CXC-R4
• CXCR-4
• D2S201E
• D2S201ESDF-1 receptor
• FB22
• Fusin
• HM89
• HM89NPYRL
• HSY3RR
• LAP3
• LAP-3
• LCR1
• LESTR
• LESTRCD184 antigen
• Leukocyte-derived seven transmembrane domain receptor
• leukocyte-derived seven-transmembrane-domain receptor
• lipopolysaccharide-associated protein 3
• neuropeptide Y receptor Y3
• NPY3R
• NPY3RWHIM
• NPYR
• NPYRL
• NPYY3R
• pCXCR4
• seven transmembrane helix receptor
• seven-transmembrane-segment receptor, spleen
• Stromal cell-derived factor 1 receptor
• WHIMS

Background

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).

1. Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
2. Zagzag, D. et al. (2005) Cancer Res. 65:6178.
3. Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
4. Wang, L. et al. (2009) Oncology Reports 22:1333.
5. Amara, S. et al. (2015) Cancer Biomark. 15:869.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR4 Antibody (MAB170)(42)

Publications using MAB170

• WG Lesniak, BB Azad, S Chatterjee, A Lisok, MG Pomper An Evaluation of CXCR4 Targeting with PAMAM Dendrimer Conjugates for Oncologic Applications Pharmaceutics, 2022-03-16;14(3):. 2022-03-16 [PMID: 35336029] (Flow Cytometry, Human)
• G Cantini, L Fei, L Canu, E Lazzeri, M Sottili, M Francalanc, ML Angelotti, G De Filpo, T Ercolino, S Gelmini, M Mangoni, G Nesi, C Hantel, M Mannelli, M Maggi, M Luconi Stimulated Expression of CXCL12 in Adrenocortical Carcinoma by the PPARgamma Ligand Rosiglitazone Impairs Cancer Progression Journal of personalized medicine, 2021-10-27;11(11):. 2021-10-27 [PMID: 34834449] (IHC, Human)
• M Armani-Tou, Z Zhou, R Gasser, I Staropoli, V Cantaloube, Y Benureau, J Garcia-Per, M Pérez-Olme, V Lorin, B Puissant-L, L Assoumou, C Delaugerre, JD Lelièvre, Y Lévy, H Mouquet, G Martin-Blo, J Alcami, F Arenzana-S, J Izopet, P Colin, B Lagane Mechanisms of HIV-1 evasion to the antiviral activity of chemokine CXCL12 indicate potential links with pathogenesis PloS Pathogens, 2021-04-19;17(4):e1009526. 2021-04-19 [PMID: 33872329] (Binding Assay, Human)
• C Ieranò, C D'Alterio, S Giarra, M Napolitano, G Rea, L Portella, A Santagata, AM Trotta, A Barbieri, V Campani, A Luciano, C Arra, AM Anniciello, G Botti, L Mayol, G De Rosa, R Pacelli, S Scala CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells Cell Death Dis, 2019-07-22;10(8):562. 2019-07-22 [PMID: 31332163] (ICC, Human)
• S Li, KW Fong, G Gritsina, A Zhang, JC Zhao, J Kim, A Sharp, W Yuan, C Aversa, XJ Yang, PS Nelson, FY Feng, AM Chinnaiyan, JS de Bono, C Morrissey, MB Rettig, J Yu Activation of MAPK signaling by CXCR7 leads to enzalutamide resistance in prostate cancer Cancer Res., 2019-04-05;0(0):. 2019-04-05 [PMID: 30952632] (Western Blot, Human)
• BA Dinkel, KN Kremer, MR Rollins, MJ Medlyn, KE Hedin GRK2 mediates TCR-induced transactivation of CXCR4 and TCR-CXCR4 complex formation which drives PI3K?/PREX1 signaling and T cell cytokine secretion J. Biol. Chem., 2018-07-17;0(0):. 2018-07-17 [PMID: 30018141] (Flow Cytometry, Human)
• SC Mills, L Howell, A Beekman, L Stokes, A Mueller Rac1 plays a role in CXCL12 but not CCL3-induced chemotaxis and Rac1 GEF inhibitor NSC23766 has off target effects on CXCR4 Cell. Signal., 2017-10-16;42(0):88-96. 2017-10-16 [PMID: 29050986] (Flow Cytometry, Human)
• Hirohito Minami, Keiki Nagaharu, Yoshiki Nakamori, Kohshi Ohishi, Naoshi Shimojo, Yuki Kageyama et al. CXCL12–CXCR4 Axis Is Required for Contact-Mediated Human B Lymphoid and Plasmacytoid Dendritic Cell Differentiation but Not T Lymphoid Generation The Journal of Immunology 2017-10-01 [PMID: 28842468]
• WL Wu, CR Grotefend, MT Tsai, YL Wang, V Radic, H Eoh, IC Huang ?20 IFITM2 differentially restricts X4 and R5 HIV-1 Proc. Natl. Acad. Sci. U.S.A., 2017-06-19;114(27):7112-7117. 2017-06-19 [PMID: 28630320] (Flow Cytometry, ICC, Human)
• Y Han, C Wu, J Wang, N Liu CXCR7 maintains osteosarcoma invasion after CXCR4 suppression in bone marrow microenvironment Tumour Biol., 2017-05-01;39(5):1010428317701. 2017-05-01 [PMID: 28468584] (Neutralization, Human)
• Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices Proc Natl Acad Sci USA, 2016-08-19;0(0):. 2016-08-19 [PMID: 27543332] (IHC-Fr, Human)
• HEREGULIN/ErbB3 SIGNALING ENHANCES CXCR4-DRIVEN Rac1 ACTIVATION AND BREAST CANCER CELL MOTILITY VIA HIF-1? Mol Cell Biol, 2016-07-14;0(0):. 2016-07-14 [PMID: 27185877] (Neutralization Control, Human)
• K Griffiths, O Dolezal, B Cao, SK Nilsson, HB See, KD Pfleger, M Roche, PR Gorry, A Pow, K Viduka, K Lim, BG Lu, DH Chang, TA Murray-Rus, M Kvansakul, MA Perugini, C Dogovski, M Doerflinge, Y Zhang, K Parisi, JL Casey, SD Nuttall, M Foley I-bodies: human single domain antibodies that antagonize chemokine receptor CXCR4 J Biol Chem, 2016-04-01;0(0):. 2016-04-01 [PMID: 27036939] (Bioassay, Human)
• Patrussi L, Capitani N, Martini V, Pizzi M, Trimarco V, Frezzato F, Marino F, Semenzato G, Trentin L, Baldari C Enhanced Chemokine Receptor Recycling and Impaired S1P1 Expression Promote Leukemic Cell Infiltration of Lymph Nodes in Chronic Lymphocytic Leukemia. Cancer Res, 2015-08-17;75(19):4153-63. 2015-08-17 [PMID: 26282174] (Flow Cytometry, Human)
• Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines. PLoS ONE, 2015-03-16;10(3):e0120069. 2015-03-16 [PMID: 25774696] (Neutralization, Human)
• Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann I, Levine B, June C, Zhang L, Wang E, Marincola F, Bedognetti D, Powell D, Tanyi J, Feldman M, Kandalaft L, Coukos G The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy. Clin Cancer Res, 2015-02-23;21(12):2840-50. 2015-02-23 [PMID: 25712684] (Neutralization, Human)
• Lin Y, Yet S, Hsu Y, Wang G, Hung S Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function. Stem Cells Transl Med, 2014-12-10;4(1):44-55. 2014-12-10 [PMID: 25504897] (Neutralization, Human)
• Coggins N, Trakimas D, Chang S, Ehrlich A, Ray P, Luker K, Linderman J, Luker G CXCR7 controls competition for recruitment of beta-arrestin 2 in cells expressing both CXCR4 and CXCR7. PLoS ONE, 2014-06-04;9(6):e98328. 2014-06-04 [PMID: 24896823] (Flow Cytometry, Human)
• Young H. Choi, Marie D. Burdick, Brett A. Strieter, Borna Mehrad, Robert M. Strieter CXCR4, but not CXCR7, Discriminates Metastatic Behavior in Non–Small Cell Lung Cancer Cells Molecular Cancer Research 2014-01-01 [PMID: 24025971]
• Park B, Kook S, Lee S, Jeong J, Brufsky A, Lee B An isoform of C/EBPbeta, LIP, regulates expression of the chemokine receptor CXCR4 and modulates breast cancer cell migration. J Biol Chem, 2013-08-21;288(40):28656-67. 2013-08-21 [PMID: 23966000] (Neutralization, Human)
• Rajareddy Singareddy, Louie Semaan, M. Katie Conley-LaComb, Jason St. John, Katelyn Powell, Matthew Iyer et al. Transcriptional regulation of CXCR4 in prostate tumor cells: Significance of TMPRSS2-ERG fusions.¶ Molecular Cancer Research 2013-11-01 [PMID: 23918819] (Immunohistochemistry, Human)
• Cédric de Poorter, Kevin Baertsoen, Vincent Lannoy, Marc Parmentier, Jean-Yves Springael Consequences of ChemR23 Heteromerization with the Chemokine Receptors CXCR4 and CCR7 PLoS ONE 2013-02-28 [PMID: 23469143]
• Dustin N D Lippert, John A Wilkins Glia maturation factor gamma regulates the migration and adherence of human T lymphocytes BMC Immunology 2012-12-01 [PMID: 22510515]
• Sun Jin Baek, Sung Keun Kang, Jeong Chan Ra In vitro migration capacity of human adipose tissue-derived mesenchymal stem cells reflects their expression of receptors for chemokines and growth factors Experimental and Molecular Medicine 2011-10-31 [PMID: 21847008]
• M Fareh, L Turchi, V Virolle, D Debruyne, F Almairac, S de-la-Forest Divonne et al. The miR 302-367 cluster drastically affects self-renewal and infiltration properties of glioma-initiating cells through CXCR4 repression and consequent disruption of the SHH-GLI-NANOG network Cell Death & Differentiation 2012-02-01 [PMID: 21720384] (Neutralization, Human, Xenograft)
• Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer. Clin. Cancer Res., 2011-04-11;17(11):3619-30. 2011-04-11 [PMID: 21482691] (Neutralization, Human)
• Rahimi Massod, George Jessica, Tang Careen et al. EGFR variant-mediated invasion by enhanced CXCR4 expression through transcriptional and post-translational mechanisms. International Journal of Cancer 1/1/2010 [PMID: 19830694]
• Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008-12-01;181(11):8053-67. 2008-12-01 [PMID: 19017998] (Flow Cytometry, ICC, IHC-P, Human)
• Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells. Exp. Hematol., 2008-06-17;36(9):1121-31. 2008-06-17 [PMID: 18562079] (Neutralization, Human)
• Kaminski A, Ma N, Donndorf P, Lindenblatt N, Feldmeier G, Ong LL, Furlani D, Skrabal CA, Liebold A, Vollmar B, Steinhoff G Endothelial NOS is required for SDF-1alpha/CXCR4-mediated peripheral endothelial adhesion of c-kit+ bone marrow stem cells. Lab. Invest., 2007-11-26;88(1):58-69. 2007-11-26 [PMID: 18040270] (Neutralization, Mouse)
• Ao M, Franco OE, Park D, Raman D, Williams K, Hayward SW Cross-talk between paracrine-acting cytokine and chemokine pathways promotes malignancy in benign human prostatic epithelium. Cancer Res., 2007-05-01;67(9):4244-53. 2007-05-01 [PMID: 17483336] (Neutralization, Human)
• McLean AB, D&amp;apos;Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Stem Cells, 2007-01-01;25(1):29-38. 2007-01-01 [PMID: 17204604] (Flow Cytometry, Human)
• Deiva K, Khiati A, Hery C, Salim H, Leclerc P, Horellou P, Tardieu M CCR5-, DC-SIGN-dependent endocytosis and delayed reverse transcription after human immunodeficiency virus type 1 infection in human astrocytes. AIDS Res. Hum. Retroviruses, 2006-11-01;22(11):1152-61. 2006-11-01 [PMID: 17147503] (Neutralization, Human)
• Lisignoli G, Cristino S, Piacentini A, Cavallo C, Caplan AI, Facchini A Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54. J. Cell. Physiol., 2006-05-01;207(2):364-73. 2006-05-01 [PMID: 16331675] (ICC, Human)
• Narducci MG, Scala E, Bresin A, Caprini E, Picchio MC, Remotti D, Ragone G, Nasorri F, Frontani M, Arcelli D, Volinia S, Lombardo GA, Baliva G, Napolitano M, Russo G Skin homing of Sezary cells involves SDF-1-CXCR4 signaling and down-regulation of CD26/dipeptidylpeptidase IV. Blood, 2005-10-04;107(3):1108-15. 2005-10-04 [PMID: 16204308] (IHC, Human)
• Zhang Y, Foudi A, Geay JF, Berthebaud M, Buet D, Jarrier P, Jalil A, Vainchenker W, Louache F Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells. Stem Cells, 2004-01-01;22(6):1015-29. 2004-01-01 [PMID: 15536192] (Flow Cytometry, Human)
• Lawrence DM, Durham LC, Schwartz L, Seth P, Maric D, Major EO Human immunodeficiency virus type 1 infection of human brain-derived progenitor cells. J. Virol., 2004-07-01;78(14):7319-28. 2004-07-01 [PMID: 15220405] (Flow Cytometry, ICC, Human)
• Tavor S, Petit I, Porozov S, Avigdor A, Dar A, Leider-Trejo L, Shemtov N, Deutsch V, Naparstek E, Nagler A, Lapidot T CXCR4 regulates migration and development of human acute myelogenous leukemia stem cells in transplanted NOD/SCID mice. Cancer Res., 2004-04-15;64(8):2817-24. 2004-04-15 [PMID: 15087398] (ICC, IHC-P, Neutralization, Human)
• Schneider P, Vasse M, Al Bayati A, Lenormand B, Vannier JP Is high expression of the chemokine receptor CXCR-4 of predictive value for early relapse in childhood acute lymphoblastic leukaemia? Br. J. Haematol., 2002-11-01;119(2):579-80. 2002-11-01 [PMID: 12406108] (ICC, Human)
• Bug G, Rossmanith T, Henschler R, Kunz-Schughart LA, Schroder B, Kampfmann M, Kreutz M, Hoelzer D, Ottmann OG Rho family small GTPases control migration of hematopoietic progenitor cells into multicellular spheroids of bone marrow stroma cells. J. Leukoc. Biol., 2002-10-01;72(4):837-45. 2002-10-01 [PMID: 12377954] (ICC, Human)
• Honczarenko M, Le Y, Glodek AM, Silberstein LE CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor. Blood, 2002-10-01;100(7):2321-9. 2002-10-01 [PMID: 12239139] (Flow Cytometry, Human)
• Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H Expression of functional CCR and CXCR chemokine receptors in podocytes. J. Immunol., 2002-06-15;168(12):6244-52. 2002-06-15 [PMID: 12055238] (IHC-Fr, Human)

FAQs for CXCR4 Antibody (MAB170). (Showing 1 - 1 of 1 FAQs).

1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.  ..show answer..
   • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Bioinformatics

• Gene Symbol: CXCR4
• Uniprot:
  • P61073()