Cyclin E1 [p Thr77] Antibody (SD2025)

Immunocytochemistry/Immunofluorescence: Cyclin E1 [p Thr77] Antibody (SD2025) [NBP2-67760] - Staining Phospho-Cyclin E1(T77) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cyclin E1 [p Thr77] Antibody (SD2025) [NBP2-67760] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin E1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/Immunofluorescence: Cyclin E1 [p Thr77] Antibody (SD2025) [NBP2-67760] - Staining Phospho-Cyclin E1(T77) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Cyclin E1 [p Thr77] Antibody (SD2025) [NBP2-67760] - Staining Phospho-Cyclin E1(T77) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cyclin E1 [p Thr77] Antibody (SD2025) [NBP2-67760] - Analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Cyclin E1(T77) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cyclin E1 [p Thr77] Antibody (SD2025) [NBP2-67760] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Cyclin E1(T77) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cyclin E1 [p Thr77] Antibody (SD2025) [NBP2-67760] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Cyclin E1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Cyclin E1 [p Thr77] Antibody (SD2025) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr77 of Human Cyclin E1 aa 51-100 / 410. (SwissProt: P24864 Human)
• Modification: p Thr77
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CCNE1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cyclin E1 [p Thr77] Antibody (SD2025)

• CCNE
• CCNE1
• CCNEcyclin Es
• Cyclin E1
• cyclin Et
• G1/S-specific cyclin-E1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCNE1