Human Cytochrome c ELISA Kit (Colorimetric)

Human Cytochrome c ELISA Kit [NBP1-91263] - Data for Human Cytochrome c Platinum ELISA

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture lysates
• Standard Curve Range: 0.08 - 5.0 ng/mL
• Sensitivity: 0.05 ng/mL

Human Cytochrome c ELISA Kit (Colorimetric) Summary

• Description: : The human Cytochrome c ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Cytochrome c.
• Specificity: The cross reactivity of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human Cytochrome c positive sample. There was no cross reactivity detected.
• Standard Curve Range: 0.08 - 5.0 ng/mL
• Sensitivity: 0.05 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 4.00%
• Intra-Assay: CV% < 6.00%
• Spike Recovery: The recovery ranged from 78% to 88% with an overall mean recovery of 82%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 100 uL (prediluted)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CYCS

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Cytochrome c ELISA Kit (Colorimetric)

• CYCHCS
• CYCS
• Cytochrome c
• cytochrome c, somatic
• THC4

Background

Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells. Mitochrondrial cytochrome c is a water-soluble protein of 15 kDa with a net positive charge, residing loosely attached in the mitochrondrial intermembrane space. Cytochrome c functions in the respiratory chain by interaction with redox partners. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of cytochrome c from mitochondria. Measurement of cytochrome c release from the mitochondria is a tool to detect the first early steps for initiating apoptosis in cells. Moreover, elevated cytochrome c levels were observed in serum from patients with hematological malignancies. In the course of cancer chemotherapy, the serum-cytochrome c level grew rapidly and it decreased gradually as the patient was cleared from malignant cells. Thus, serum-cytochrome c monitoring might serve as a clinical marker indicating the onset of apoptosis and cell turn-over in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CYCS