Cytochrome c Apoptosis Detection Kit

Product Discontinued

Cytochrome c Apoptosis Detection Kit Summary

• Description: Convenient and effective assay for detecting cytochrome c translocation from mitochondria into the cytosol during apoptosis. No need to ultracentrifuge.
  
  Detection method: Western blot.
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65311)
• Kit Type: Apoptosis Detection Kit
• Gene: CYCS

Applications/Dilutions

• Application Notes: Cytochrome c release from mitochondria into the cytosol is determined by Western blotting using the cytochrome c antibody provided in the kit.

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Cytochrome c Apoptosis Detection Kit

• CYCHCS
• CYCS
• Cytochrome c
• cytochrome c, somatic
• THC4

Background

Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. The Cytochrome c Apoptosis Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CYCS