Cytochrome C Oxidase Native Protein

• Reactivity: Bv
• Applications: Func, Bioactivity
• Concentration: 5 mg/ml

Cytochrome C Oxidase Native Protein Summary

• Description: Native biologically active Cytochrome C Oxidase.

  Source: Bovine heart

• Source: Bovine heart
• Protein/Peptide Type: Native Protein
• Purity: >50% SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • In vitro assay
• Application Notes: Cytochrome c Oxidase / Complex IV consists of 7-13 different proteins and is isolated as a dimer of 250 kDa of which heme a is an integral part. It contains macromolecular complex IV associated with micelles of the detergent n-dodecyl beta-D-maltoside and the enzyme solubilized with n-dodecyl beta-D-maltoside has an apparent molecular mass of 300-350 kDa (gel filtration). Cytochrome C Oxidase from Bovine heart. Activity: > or =20 units/mg protein (Unit: 1 unit of cytochrome c oxidase oxidizes 1 umole of ferrocytochrome c per minute at 25C at pH 6)
• Theoretical MW: 250 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 25 mM Tris-HCl buffer, pH 7.8, 5 mM EDTA, and 39 mM n-dodecyl beta-D-maltoside
• Preservative: No Preservative
• Concentration: 5 mg/ml
• Purity: >50% SDS-PAGE

Alternate Names for Cytochrome C Oxidase Native Protein

• Complex IV
• EC 1.9.3.1
• Ferrocytochrome-c:oxygen oxidoreductase

Background

Cytochrome c oxidase is the principal terminal oxidase of high oxygen affinity in the aerobic metabolism of all animals, plants, yeasts and some bacteria. It is present in the mitochondria of the more highly developed cells and in the cytoplasmic membrane of bacteria. Cytochrome c oxidase catalyses the electron transfer from cytochrome c to O2. This electron-transfer process produces a proton gradient across the membrane, which in turn drives the production of ATP. This enzyme is unique in providing energy for the cell bycoupling the electron transport through the cytochrome chain with the process of oxidative phosphorylation. Cytochrome c Oxidase (CAS RN 9001-16-5) is also called Complex IV, Ferrocytochrome-c:oxygen oxidoreductase and EC 1.9.3.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.