Cytochrome C Oxidase subunit 6c Antibody

Western Blot: Cytochrome C Oxidase subunit 6c Antibody [NBP2-97924] - Anti-Cytochrome C Oxidase subunit 6c rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: A673 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 9 kDa. Observed band size: 11 kDa

Immunocytochemistry/Immunofluorescence: Cytochrome C Oxidase subunit 6c Antibody [NBP2-97924] - Immunofluorescence staining of Cytochrome C Oxidase subunit 6c in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Cytochrome C Oxidase subunit 6c polyclonal antibody (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunohistochemistry-Paraffin: Cytochrome C Oxidase subunit 6c Antibody [NBP2-97924] - Immunochemical staining of human Cytochrome C Oxidase subunit 6c in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: Cytochrome C Oxidase subunit 6c Antibody [NBP2-97924] - Immunochemical staining of human Cytochrome C Oxidase subunit 6c in human duodenum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Product Discontinued

Cytochrome C Oxidase subunit 6c Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human Cytochrome C Oxidase subunit 6c fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COX6C
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0)
• Preservative: 0.03% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Cytochrome C Oxidase subunit 6c Antibody

• Cytochrome c oxidase polypeptide VIc
• cytochrome c oxidase subunit 6C
• cytochrome c oxidase subunit VIc preprotein
• cytochrome c oxidase subunit VIc

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COX6C