Recombinant Human Cytochrome P450 2E1 His Protein

SDS-Page: Cytochrome P450 2E1 Recombinant Protein [NBP2-51999] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human Cytochrome P450 2E1 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 29-493 of Human Cytochrome P450 2E1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSWNLPPG PFPLPIIGNL FQLELKNIPK SFTRLAQRFG PVFTLYVGSQ RMVVMHGYKA VKEALLDYKD EFSGRGDLPA FHAHRDRGII FNNGPTWKDI RRFSLTTLRN YGMGKQGNES RIQREAHFLL EALRKTQGQP FDPTFLIGCA PCNVIADILF RKHFDYNDEK FLRLMYLFNE NFHLLSTPWL QLYNNFPSFL HYLPGSHRKV IKNVAEVKEY VSERVKEHHQ SLDPNCPRDL TDCLLVEMEK EKHSAERLYT MDGITVTVAD LFFAGTETTS TTLRYGLLIL MKYPEIEEKL HEEIDRVIGP SRIPAIKDRQ EMPYMDAVVH EIQRFITLVP SNLPHEATRD TIFRGYLIPK GTVVVPTLDS VLYDNQEFPD PEKFKPEHFL NENGKFKYSD YFKPFSTGKR VCAGEGLARM ELFLLLCAIL QHFNLKPLVD PKDIDLSPIH IGFGCIPPRY KLCVIPRS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CYP2E1
• Purity: >80%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 56.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >80%, by SDS-PAGE

Alternate Names for Recombinant Human Cytochrome P450 2E1 His Protein

• 4-nitrophenol 2-hydroxylase
• CPE1
• CYP2Ecytochrome P450 2E1
• CYPIIE1
• cytochrome P450, family 2, subfamily E, polypeptide 1
• cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1
• Cytochrome P450-J
• EC 1.14.13.-
• EC 1.14.13.n7
• EC 1.14.14.1
• flavoprotein-linked monooxygenase
• microsomal monooxygenase
• P450C2E
• P450-J
• xenobiotic monooxygenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CYP2E1