Cytokeratin 7 Antibody (CK-7)

Product Discontinued

Cytokeratin 7 Antibody (CK-7) Summary

• Immunogen: Cytoskeletal preparation of the RT4human bladder carcinoma cell line.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT7
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 2-4 ug/ml
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1-2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1.2% Sodium Acetate and 0.2 mg BSA
• Preservative: 0.01 mg Sodium Azide
• Concentration: LYOPH
• Purity: Protein A or G purified
• Reconstitution Instructions: Reconstitute with deionized water.

Alternate Names for Cytokeratin 7 Antibody (CK-7)

• CK7
• CK-7
• Cytokeratin 7
• cytokeratin-7
• K2C7
• K7
• K7keratin, 55K type II cytoskeletal
• keratin 7
• keratin, type II cytoskeletal 7
• keratin-7
• MGC129731
• MGC3625
• sarcolectin
• SCLkeratin, simple epithelial type I, K7
• type II mesothelial keratin K7
• Type-II keratin Kb7

Background

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT7
• Entrez:
  • 3855(Human)
• Uniprot:
  • P08729(Human)